Anti-Mouse CD25 In Vivo Antibody – Low Endotoxin (PC61) [ICH1054]

ichorbio’s anti-Mouse CD25 In Vivo Antibody – Low Endotoxin (PC61) is manufactured in a cGMP compliant, ISO Quality Standard 9001:2015 facility. ichorbio’s low endotoxin antibodies have half the endotoxin of comparable antibodies from our competitors at less than 1.0 EU/mg. If ichorbio’s low endotoxin antibodies are not low enough we also offer ultra low endotoxin antibodies which have even less endotoxin (<0.75EU/mg) at an even higher purity (98% versus 95%). ichorbio offers Amazon vouchers or donations to the NC3Rs for reviews of this product: click here for more information. ichorbio: the best antibodies for in vivo research.

CD25

PC61

Rat IgG1

Interleukin-2 receptor subunit alpha, IL-2 receptor subunit alpha, IL-2-RA, IL-2R subunit alpha, IL2-RA, Il2ra, p55

Rat

Mouse

Anti-CD25 In Vivo Antibody – Low Endotoxin (PC61) recognizes an epitope on Mouse IL-2 RA

This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

The CD25 antigen is detected on CD4 positive T-cells but not on CD8 positive T-cells. However, when stimulated with phytohemagglutinin (PHA), the CD25 antigen can be detected on CD8 positive T cells. The CD25 antigen is also expressed on CD20 positive B cells. The CD25 antigen is negative on CD 56 positive natural killer cells, CD 14 positive monocytes, granulocytes, red blood cells and platlets. The CD25 antigen is expressed on malignant cells of adult T-cell leukemias (ATL’s) carring HTLV-1, most cases of cutaneous T cell lymphoma and lymphoepithelioid lymphomas among non-Hodgkin’s T cell lymphomas.

IL-2 receptor alpha chain (IL-2Ralpha) is a component of high-affinity IL-2 receptors and thus is a key regulator of lymphocyte proliferation. IL-2R alpha expression occurs at specific stages of early T and B lymphocyte development and is induced upon activation of mature lymphocytes. IL-2R alpha is essential for regulation of both the size and content of the peripheral lymphoid compartment, probably by influencing the balance between clonal expansion and cell death following lymphocyte activation. IL-2Ralpha is also important in the formation of high-affinity binding sites, and the inducibility of the alpha gene contributes to the highly regulated and transient display of high-affinity IL-2R.

IL-2-dependent cytolytic mouse T-cell clone B6.1

1.0 – 5.0 mg/ml

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.

>95% by SDS-PAGE and HPLC

>98% by SDS-PAGE and HPLC

≤ 1.0 EU/mg as determined by the LAL method

≤ 0.75 EU/mg as determined by the LAL method

Aggregation level ≤ 5%

Aggregation level ≤ 1%

This antibody is stable for at least 4 weeks when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 20°C or -80°C. Avoid repeated freeze thaw cycles.

Immunoprecipitation, Western Blot, Functional Assays, Blocking, Flow Cytometry, IHC (Frozen)

Each investigator should determine their own optimal working dilution for specific applications.

Products are for research use only. Not for use in diagnostic or therapeutic procedures.