Anti-Mouse 4-1BBL In Vivo Antibody – Low Endotoxin (TKS-1) [ICH1184]

ichorbio’s Anti-Mouse 4-1BBL In Vivo Antibody – Low Endotoxin (TKS-1) [ICH1184] is manufactured in a cGMP compliant, ISO Quality Standard 9001:2015 facility. ichorbio’s low endotoxin antibodies have half the endotoxin of comparable antibodies from our competitors at less than 1.0 EU/mg. If ichorbio’s low endotoxin antibodies are not low enough we also offer ultra low endotoxin antibodies which have even less endotoxin (<0.75EU/mg) at an even higher purity (98% versus 95%). ichorbio: the best antibodies for in vivo research.

4-1BBL

TKS-1

ichorbio’s TKS-1 in vivo antibody is available in the following bulk sizes: 5mg, 25mg, 50mg and 100mg. ichorbio regularly manufactures multi-gram amounts of our anti-4-1BBL TKS-1 clone – please contact us for pricing.

IgG2a kappa

CD137L, TNFSF9, 4-1BB Ligand, TKS-1

Rat

Mouse

4-1BBL is expressed by activated B cells, macrophages, and dendritic cells.

4-1BBL (CD137L) and 4-1BB (CD137) are a receptor ligand pair belonging to the tumor necrosis factor (TNF) receptor/TNF superfamily. 4-1BBL is a type II transmembrane protein expressed by activated B cells, macrophages, and dendritic cells. Members of the TNF-TNF receptor superfamily play critical roles in regulating cellular activation, differentiation, and apoptosis and the 4-1BBL-4-1BB interaction is important in cellular immune responses. 4-1BBL-4-1BB interaction provides a co-stimulatory signal to T cells, resulting in increased proliferation and cytokine production. A TKS-1-producing hybridoma was generated by immunizing a SD rat with 4-1BBL-transfected NRK cells (rat IgG2a,κ). Splenocytes were fused with P3U1 cells and hypoxanthine–aminopterin–thymidine selection was performed. The monoclonal antibody was identified by its strong reactivity with 4-1BBL-transfected L5178Y cells, cloned by limiting diffusion, purified from ascites, and found to bind to 4-1BBL/NRK and 4-1BBL/P815 cells. Pre-incubation with TKS-1 blocks 4-1BB–Ig binding to 4-1BBL/L5178Y cells, indicating TKS-1 is specific to mouse 4-1BBL and can interrupt the interaction between 4-1BBL and 4-1BB. Additionally, TKS-1 can block anti-CD3-stimulated T cell proliferation co-stimulated by 4-1BBL /P815 cells. The monoclonal antibodies 19H3 and TKS-1 bind to different sites on murine 4-1BBL, with TKS-1 binding to a site that overlaps with the receptor binding site. Additionally, TKS-1 and 4-1BB bind to a similar site, making TKS-1 especially useful for blocking studies.

1.0 – 5.0 mg/ml

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.

>95% by SDS-PAGE and HPLC

>98% by SDS-PAGE and HPLC

<1.0 EU/mg as determined by the LAL method

≤ 0.75 EU/mg as determined by the LAL method

Aggregation level ≤ 5%

Aggregation level ≤ 1%

Anti-4-1BBL In Vivo Antibody – Low Endotoxin (TKS-1) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

Functional Assays, Blocking, Western Blot

Each investigator should determine their own optimal working dilution for specific applications..

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

Rat IgG2a In Vivo Isotype Control – Low Endotoxin (1-1) [ICH2244]