Anti-Human MHC Class I In Vivo Antibody – Low Endotoxin (W6/32) [ICH1023]

ichorbio’s anti-MHC Class I In Vivo Antibody – Low Endotoxin (W6/32) is manufactured in a cGMP compliant, ISO Quality Standard 9001:2015 facility. ichorbio’s low endotoxin antibodies have half the endotoxin of comparable antibodies from our competitors at less than 1.0 EU/mg. If ichorbio’s low endotoxin antibodies are not low enough we also offer ultra low endotoxin antibodies which have even less endotoxin (<0.75EU/mg) at an even higher purity (98% versus 95%). ichorbio: the best antibodies for in vivo research.

MHC Class I

W6/32

ichorbio’s W6/32 in vivo antibody is available in the following bulk sizes:
1mg, 5mg, 25mg, 50mg and 100mg
ichorbio regularly manufactures multi-gram amounts of our anti-MHC Class I W6/32 clone – please contact us for pricing.

Mouse IgG2a

Major Histocompatibility Class I, MHC class I

Mouse

Human

Anti-MHC Class I In Vivo Antibody – Low Endotoxin (W6/32) recognizes/reacts with major histocompatibility complex (MHC) class I, HLA-A, B, and C

This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

This HLA-A, B, C antigen is present on most nucleated cells of humans and old world monkeys.

MHC class I antigens associated with β2-microglobulin are expressed by all human nucleated cells. MHC class I molecules are involved in the presentation of antigens to CD8+ T cells and plays a central role in cell-mediated immune responses and tumor surveillance.

Human tonsil cell membrane

1.0 mg/ml

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.

>95% by SDS-PAGE and HPLC

>98% by SDS-PAGE and HPLC

≤ 1.0 EU/mg as determined by the LAL method

≤ 0.75 EU/mg as determined by the LAL method

Aggregation level ≤ 5%

Aggregation level ≤ 1%

anti-MHC Class I In Vivo Antibody – Low Endotoxin (W6/32) is stable for at least six (6) weeks when stored sterile at 2-8°C. For long-term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

Western Blot, Flow Cytometry, Immunoprecipitation, Functional Assays, IHC (Frozen)

Each investigator should determine their own optimal working dilution for specific applications.

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

Mouse IgG2a In Vivo Isotype Control – Low Endotoxin [C1.18.4] (ICH2245)

Anti-MHC Class I In Vivo Antibody – Low Endotoxin [A4C8.1-Do9] (ICH1097), Anti-MHC Class I In Vivo Antibody – Low Endotoxin [AF6-88.5] (ICH1098)