Anti-Mouse Ly-6G In Vivo Antibody – Low Endotoxin (1A8) [ICH1089]

ichorbio’s anti-Ly-6G In Vivo Antibody – Low Endotoxin (1A8) is manufactured in a cGMP compliant facility. ichorbio’s low endotoxin antibodies have half the endotoxin of comparable antibodies from our competitors at less than 1.0 EU/mg. If ichorbio’s low endotoxin antibodies are not low enough we also offer ultra low endotoxin antibodies which have even less endotoxin (<0.75EU/mg) at an even higher purity (98% versus 95%). ichorbio: the best antibodies for in vivo research.

Ly-6G

1A8

Rat IgG2a

Lymphocyte antigen 6 complex, locus G

Rat

Mouse

Anti-Ly-6G In Vivo Antibody – Low Endotoxin (1A8) recognizes an epitope on Mouse Ly-6G. Unlike clone RB6-8C5 antibody, the 1A8 antibody reacts specifically with mouse Ly6G with no reported cross-reactivity with Ly6C

This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes.

Ly-6G is a 21-25 kD GPI-anchored protein, is expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes.

Mouse Ly-6G transfected EL-4J cell line

1.0 mg/ml

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.

>95% by SDS-PAGE and HPLC

>98% by SDS-PAGE and HPLC

≤ 1.0 EU/mg as determined by the LAL method

≤ 0.75 EU/mg as determined by the LAL method

Aggregation level ≤ 5%

Aggregation level ≤ 1%

This antibody is stable for at least 4 weeks when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 20°C or -80°C. Avoid repeated freeze thaw cycles.

IHC (FFPE), IHC (Frozen), Flow Cytometry, Western Blot, In vivo Depletion, Immunfluorescence

Each investigator should determine their own optimal working dilution for specific applications.

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

Rat IgG2a In Vivo Isotype Control – Low Endotoxin [1-1] (ICH2244)

Anti-Ly-6G/Ly-6C In Vivo Antibody – Low Endotoxin [RB6-8C5] (ICH1131), Anti-Ly-6G/Ly-6C In Vivo Antibody – Ultra Low Endotoxin [RB6-8C5] (ICH1131UL), Anti-Ly-6C In Vivo Antibody – Low Endotoxin [HK1.4] (ICH1111), Anti-Ly-6C In Vivo Antibody – Low Endotoxin [7B10] (ICH1095)

Immunofluorescence analysis of paraffin-embedded mouse spleen tissue section labeling Ly6g (1:100 dilution) overnight at 4˚, followed by goat anti-mouse IgG H&L (Alexa Fluor ® 647-red) secondary antibody (1:500 dilution). The nuclear counter stain is DAPI (blue). Image was acquired on a Nikon A1R microscope system at 4x magnification (100um panel) or 60x magnification (20um panel). Images provided by Binaree, Inc.