Anti-Mouse Ly-6C In Vivo Antibody – Low Endotoxin (HK1.4) [ICH1111]

ichorbio’s anti-Ly-6C In Vivo Antibody – Low Endotoxin (HK1.4) is manufactured in a cGMP compliant, ISO Quality Standard 9001:2015 facility. ichorbio’s low endotoxin antibodies have half the endotoxin of comparable antibodies from our competitors at less than 1.0 EU/mg. If ichorbio’s low endotoxin antibodies are not low enough we also offer ultra low endotoxin antibodies which have even less endotoxin (<0.75EU/mg) at an even higher purity (98% versus 95%). ichorbio: the best antibodies for in vivo research.

Ly-6C

HK1.4

Rat IgG2c

Lymphocyte antigen 6 complex, locus C

Rat

Mouse

Anti-Ly-6C In Vivo Antibody – Low Endotoxin (HK1.4) recognizes an epitope on Mouse Ly-6C

This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

The antigen is found on some monocyte/macrophage populations, endothelial cells, thymocytes, NK-cells, T-cell subsets and 40% of bone marrow cells from all mouse strains tested. Clone HK1.4 does not block the binding of clone RB6-8C5

L3 cloned CTL cells

0.5 mg/ml

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.

>95% by SDS-PAGE and HPLC

>98% by SDS-PAGE and HPLC

≤ 1.0 EU/mg as determined by the LAL method

≤ 0.75 EU/mg as determined by the LAL method

Aggregation level ≤ 5%

Aggregation level ≤ 1%

This antibody is stable for at least 4 weeks when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 20°C or -80°C. Avoid repeated freeze thaw cycles.

Immunoprecipitation, Western Blot

Immunoprecipitation: 1–2 μg per 100–500 μg of total protein (1 ml of cell lysate).
Each investigator should determine their own optimal working dilution for specific applications.

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

Anti-Ly-6G/Ly-6C In Vivo Antibody – Low Endotoxin [RB6-8C5] (ICH1131), Anti-Ly-6G/Ly-6C In Vivo Antibody – Ultra Low Endotoxin [RB6-8C5] (ICH1131UL), Anti-Ly-6C In Vivo Antibody – Low Endotoxin [7B10] (ICH1095), Anti-Ly-6G In Vivo Antibody – Low Endotoxin [1A8] (ICH1089), Anti-Ly-6G In Vivo Antibody – Ultra Low Endotoxin [1A8] (ICH1089UL)