Anti-Mouse IL-4 In Vivo Antibody – Low Endotoxin (11B11) [ICH1087]

ichorbio’s anti-IL-4 In Vivo Antibody – Low Endotoxin (11B11) is manufactured in a cGMP compliant, ISO Quality Standard 9001:2015 facility. ichorbio’s low endotoxin antibodies have half the endotoxin of comparable antibodies from our competitors at less than 1.0 EU/mg. If ichorbio’s low endotoxin antibodies are not low enough we also offer ultra low endotoxin antibodies which have even less endotoxin (<0.75EU/mg) at an even higher purity (98% versus 95%). ichorbio: the best antibodies for in vivo research.

IL-4

11B11

Rat IgG1

Interleukin-4, Il-4, B-cell IgG differentiation factor, B-cell growth factor 1, B-cell stimulatory factor 1, BSF-1, IGG1 induction factor, Lymphocyte stimulatory factor 1

P07750

Rat

Mouse

Anti-IL-4 In Vivo Antibody – Low Endotoxin (11B11) recognizes an epitope on Mouse IL-4

This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Interleukin-4 (IL-4) is a pleiotropic immune cytokine secreted by activated TH2 cells that inhibits bone resorption. IL-4 is critical for inducing allergic responses. It has many biological roles, including the stimulation of activated B-cell and T-cell proliferation, and the differentiation of CD4+ T-cells into Th2 cells. It is also a key regulator in humoral and adaptive immunity. IL-4 is required for the pathogenesis of atopic diseases and immune regulation.

Purified Recombinant Mouse IL-4 (>98%)

1.0 – 5.0 mg/ml

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.

>95% by SDS-PAGE and HPLC

>98% by SDS-PAGE and HPLC

<0.5 EU/mg as determined by the LAL method

≤ 0.75 EU/mg as determined by the LAL method

Aggregation level ≤ 5%

Aggregation level ≤ 1%

This antibody is stable for at least 4 weeks when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 20°C or -80°C. Avoid repeated freeze thaw cycles.

ELISA Capture, ELISPOT, Flow Cytometry, Neutralization, Immunoprecipitation, Functional Assays, CyTOF, Immunohistochemistry

ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 0.5-2.0 µg/ml.
ELISPOT Capture: This Low Endotoxin Functional Formulation antibody has been reported for use as the capture antibody in a sandwich ELISPOT assay. Aseptically coat plates at a concentration of 2-6 µg/ml is recommended.
Neutralization: Anti-Mouse IL-4 (clone 11B11) antibody can neutralize the bioactivity of natural or recombinant IL-4. The Low Endotoxin Functional Formulation purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for neutralization of mouse IL-4 bioactivity in vivo.
Each investigator should determine their own optimal working dilution for specific applications.

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

Rat IgG1 Isotype Control for In Vivo – Low Endotoxin [GL113] (ICH2246)