Anti-Mouse IFN beta In Vivo Antibody – Low Endotoxin (MIB-5E9.1) [ICH1124]

ichorbio’s anti-IFN beta In Vivo Antibody – Low Endotoxin (MIB-5E9.1) is manufactured in a cGMP compliant, ISO Quality Standard 9001:2015 facility. ichorbio’s low endotoxin antibodies have half the endotoxin of comparable antibodies from our competitors at less than 1.0 EU/mg. If ichorbio’s low endotoxin antibodies are not low enough we also offer ultra low endotoxin antibodies which have even less endotoxin (<0.75EU/mg) at an even higher purity (98% versus 95%). ichorbio: the best antibodies for in vivo research.

IFN beta

MIB-5E9.1

Armenian Hamster IgG

IFN-b, IFN-beta, Type-1 interferon, Interferon beta

Armenian Hamster

Mouse

Anti-IFN beta In Vivo Antibody – Low Endotoxin (MIB-5E9.1) recognizes Mouse IFN beta

This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Fibroblasts & epithelial cells

Interferons (IFN) consist of two classes. The type I IFN class consists of 20 highly similar IFN-alpha proteins and a single IFN-beta protein. The second class, type II IFN, consists of a single protein, IFN-gamma. IFN-alpha and IFN-beta signal through the same cell surface receptor IFNAR1 and have a similar range of biological activities including antiviral and antiproliferative activity. The type I IFNs also influence activation, growth and differentiation of T cells, B cells, macrophages, NK cells and other cell types such as endothelial cells and fibroblasts. IFN-beta has recently proven to be beneficial in patients with multiple sclerosis and could also be a potential therapy for rheumatoid arthritis. It is unclear what cellular mechanisms are involved in IFN-beta’s therapeutic effects, but they may be caused by a restoration of balance between pro- and anti-inflammatory cytokines.

1.0 mg/ml

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.

>95% by SDS-PAGE and HPLC

>98% by SDS-PAGE and HPLC

≤ 1.0 EU/mg as determined by the LAL method

≤ 0.75 EU/mg as determined by the LAL method

Aggregation level ≤ 5%

Aggregation level ≤ 1%

anti-IFN beta In Vivo Antibody – Low Endotoxin (MIB-5E9.1) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

Immunoprecipitation, Western Blot, Neutralisation, Blocking

Each investigator should determine their own optimal working dilution for specific applications.

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

Armenian Hamster IgG Isotype Control for In Vivo – Low Endotoxin [PIP] (ICH2251)

Anti-IFN beta In Vivo Antibody – Low Endotoxin [HDB-4A7] (ICH1110), Anti-IFN beta In Vivo Antibody – Ultra Low Endotoxin [HDB-4A7] (ICH1110UL)