Bulk anti-IFN beta In Vivo Antibody – Low Endotoxin (HDB-4A7)
ichorbio’s anti-IFN beta In Vivo Antibody – Low Endotoxin (HDB-4A7) is manufactured in a cGMP compliant. ichorbio’s low endotoxin antibodies have half the endotoxin of comparable antibodies from our competitors at less than 1.0 EU/mg. If ichorbio’s low endotoxin antibodies are not low enough we also offer ultra low endotoxin antibodies which have even less endotoxin (<0.75EU/mg) at an even higher purity (98% versus 95%). ichorbio: the best antibodies for in vivo research.
ichorbio’s HDB-4A7 in vivo antibody is available in the following bulk sizes:
1mg, 5mg, 25mg, 50mg and 100mg
ichorbio regularly manufactures multi-gram amounts of our anti-interferon beta HDB-4A7 clone – please contact us for pricing.
IFN-b, IFN-beta, Type-1 interferon, Interferon beta
Anti-IFN beta In Vivo Antibody – Low Endotoxin (HDB-4A7) recognizes an epitope on Mouse IFN-beta and binds specifically to murine IFNbeta and does not bind murine IFNg or several different IFNa species (a-1,4,5 or 13); HDb-4A7 binds both recombinant and natural forms
This antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Plasmid DNA encoding murine IFNb
1.0 – 5.0 mg/ml
0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.
>95% by SDS-PAGE and HPLC
>98% by SDS-PAGE and HPLC
≤ 1.0 EU/mg as determined by the LAL method
≤ 0.75 EU/mg as determined by the LAL method
Aggregation level ≤ 5%
Aggregation level ≤ 1%
We use the IMPACT test generated by IDEXX Laboratories to guarantee our Ultra Low Endotoxin antibodies are pathogen free. Our mouse antibodies are tested for:
Mouse hepatitis virus
Pneumonia virus of mice
Minute virus of mice
Mouse parvovirus (MPV1-5)
Theiler’s murine encephalomyelitis virus
Lymphocytic choriomeningitis virus
Lactate dehydrogenase-elevating virus
Mouse adenovirus (MAD1, MAD2)
Mouse thymic virus
Corynebacterium spp. (HAC2)
This antibody is stable for at least 4 weeks when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 20°C or -80°C. Avoid repeated freeze thaw cycles.
Western Blot, Blocking, Functional Assays
Blocking: Blocks in vivo IFNb leading to increased the lethality of mice infected with West Nile Virus (WNV), similar to the susceptibility of IFNb-/- mice. Clone HDB-4A7 circulates with a half-life of ~ 14 days.
Each investigator should determine their own optimal working dilution for specific applications.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.