Anti-IFN beta In Vivo Antibody – Low Endotoxin (HDB-4A7) [ICH1110]

ichorbio’s anti-IFN beta In Vivo Antibody – Low Endotoxin (HDB-4A7) is manufactured in a cGMP compliant. ichorbio’s low endotoxin antibodies have half the endotoxin of comparable antibodies from our competitors at less than 1.0 EU/mg. If ichorbio’s low endotoxin antibodies are not low enough we also offer ultra low endotoxin antibodies which have even less endotoxin (<0.75EU/mg) at an even higher purity (98% versus 95%). ichorbio: the best antibodies for in vivo research.

IFN beta

HDB-4A7

ichorbio’s HDB-4A7 in vivo antibody is available in the following bulk sizes:
1mg, 5mg, 25mg, 50mg and 100mg
ichorbio regularly manufactures multi-gram amounts of our anti-interferon beta HDB-4A7 clone – please contact us for pricing.

Mouse IgG2a

IFN-b, IFN-beta, Type-1 interferon, Interferon beta

Mouse

Mouse

Anti-IFN beta In Vivo Antibody – Low Endotoxin (HDB-4A7) recognizes an epitope on Mouse IFN-beta and binds specifically to murine IFNbeta and does not bind murine IFNg or several different IFNa species (a-1,4,5 or 13); HDb-4A7 binds both recombinant and natural forms

This antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Plasmid DNA encoding murine IFNb

1.0 – 5.0 mg/ml

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.

>95% by SDS-PAGE and HPLC

>98% by SDS-PAGE and HPLC

≤ 1.0 EU/mg as determined by the LAL method

≤ 0.75 EU/mg as determined by the LAL method

Aggregation level ≤ 5%

Aggregation level ≤ 1%

This antibody is stable for at least 4 weeks when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 20°C or -80°C. Avoid repeated freeze thaw cycles.

Western Blot, Blocking, Functional Assays

Blocking: Blocks in vivo IFNb leading to increased the lethality of mice infected with West Nile Virus (WNV), similar to the susceptibility of IFNb-/- mice. Clone HDB-4A7 circulates with a half-life of ~ 14 days.
Each investigator should determine their own optimal working dilution for specific applications.

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

Mouse IgG2a In Vivo Isotype Control – Low Endotoxin [C1.18.4] (ICH2245)

Anti-IFN beta In Vivo Antibody – Low Endotoxin [MIB-5E9.1] (ICH1124)