Bulk anti-Human CD7 antibody (T3-3A1) [ICH1158]

ichorbio’s bulk anti-human CD7 antibody (T3-3A1) is manufactured in a cGMP compliant, ISO Quality Standard 9001:2015 facility. ichorbio’s low endotoxin antibodies have half the endotoxin of comparable antibodies from our competitors at less than 1.0 EU/mg. If ichorbio’s low endotoxin antibodies are not low enough we also offer ultra low endotoxin antibodies which have even less endotoxin (<0.75EU/mg) at an even higher purity (98% versus 95%). ichorbio: the best antibodies for in vivo research.

CD7

T3-3A1

Mouse IgG1 κ

P50283

Mouse

Mouse

Clone T3-3A1 recognizes an epitope on human CD7.

This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

CD7 is expressed on T cells, NK cells, thymocytes, hematopoietic progenitors, some normal B cells, malignant B cells, acute lymphocytic leukemia (ALL) cells, and some acute myeloid leukemia (AML) cells. It is weakly expressed on monocytes.

CD7 is a 40 kD type I transmembrane glycoprotein and is a member of the Ig superfamily. It plays a vital role in T-cell interactions, in adition to, T-cell/B-cell interaction during early lymphoid development, and has been shown to interact with PIK3R1. Functional studies indicate that crosslinking of the CD7 can stimulate transmembrane calcium flux in T cells. Lack of CD7 expression can help to diagnose T-cell lymphoproliferative disorders, such as mycosis fungoides/Sezary syndrome and adult T-cell leukemia/lymphoma.

1.0 mg/ml

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (PBS) pH 7.2 – 7.4, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and azide free

>95% by SDS-PAGE and HPLC

>98% by SDS-PAGE and HPLC

≤ 1.0 EU/mg as determined by the LAL method

≤ 0.75 EU/mg as determined by the LAL method

Aggregation level ≤ 5%

Aggregation level ≤ 1%

This antibody is stable for at least 4 weeks when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 20°C or -80°C. Avoid repeated freeze thaw cycles.

Flow cytometry, Immunofluorescence

Each investigator should determine their own optimal working dilution for specific applications.

Products are for research use only. Not for use in diagnostic or therapeutic procedures.