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Home / Applications / Flow Cytometry
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Western Blot clone EGFR.1

Bulk anti-Human EGFR antibody (EGFR.1) [ICH1021]

Rated 5.00 out of 5 based on 1 customer rating

$326.00 – $2,425.00

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Western Blot clone EGFR.1
Bulk anti-Human EGFR antibody (EGFR.1) [ICH1021]
SKU: ICH1021 Categories: anti-human, Flow Cytometry, IHC (Frozen), ImmunoCytoChemistry, Immunofluorescence, Immunoprecipitation, IP-Mass Spectrometry, Low Endotoxin, Neutralization, Ultra Low Endotoxin, Western Blot
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Bulk anti-human EGFR antibody (EGFR.1)

 

Product Benefits:

ichorbio’s bulk anti-human EGFR antibody (EGFR.1) is manufactured in a cGMP compliant facility. ichorbio’s low endotoxin antibodies have half the endotoxin of comparable antibodies from our competitors at less than 1.0 EU/mg. If ichorbio’s low endotoxin antibodies are not low enough we also offer ultra low endotoxin antibodies which have even less endotoxin (<0.75EU/mg) at an even higher purity (98% versus 95%). ichorbio offers Amazon vouchers or donations to the NC3Rs for reviews of this product: click here for more information. ichorbio: the best antibodies for in vivo research.

Target:

EGFR

Clone:

EGFR.1

Isotype:

Mouse IgG2b

Other Names:

Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, ERBB, ERBB1, HER1

Uniprot:

P00533

Host:

Mouse

Species Reactivity:

Human

Specificity:

Bulk anti-human EGFR antibody (EGFR.1) recognizes the (Mr 170 kDa) extracellular domain of the epidermal growth factor receptor on many cell surfaces. The EGFR mediates the initial response of cells to EGF. It has an extracellular region which binds EGF and an intracellular region which exhibits tyrosine kinase activity. The receptor phosphorylates a number of protein substrates in addition to mediating autophosphorylation

Background:

The epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). Mutations affecting EGFR expression or activity could result in cancer. EGFR (epidermal growth factor receptor) exists on the cell surface and is activated by binding of its specific ligands, including epidermal growth factor and transforming growth factor A (TGFA) (note, a full list of the ligands able to activate EGFR and other members of the ErbB family is given in the ErbB article). ErbB2 has no known direct activating ligand, and may be in an activated state constitutively or become active upon heterodimerization with other family members such as EGFR. Upon activation by its growth factor ligands, EGFR undergoes a transition from an inactive monomeric form to an active homodimer – although there is some evidence that preformed inactive dimers may also exist before ligand binding. In addition to forming homodimers after ligand binding, EGFR may pair with another member of the ErbB receptor family, such as ErbB2/Her2/neu, to create an activated heterodimer. There is also evidence to suggest that clusters of activated EGFRs form, although it remains unclear whether this clustering is important for activation itself or occurs subsequent to activation of individual dimers.

Immunogen:

A431 cultured cells

Concentration:

1.0 – 5.0 mg/ml

Formulation:

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.

Purity:

>95% by SDS-PAGE and HPLC

>98% by SDS-PAGE and HPLC

Endotoxin:

≤ 1.0 EU/mg as determined by the LAL method

≤ 0.75 EU/mg as determined by the LAL method

Aggregation:

Aggregation level ≤ 5%

Aggregation level ≤ 1%

IMPACT Pathogen Test:

We use the IMPACT test generated by IDEXX Laboratories to guarantee our Ultra Low Endotoxin antibodies are pathogen free. Our mouse antibodies are tested for:
Mycoplasma spp.
Mycoplasma pulmonis
Sendai virus
Mouse hepatitis virus
Pneumonia virus of mice
Minute virus of mice
Mouse parvovirus (MPV1-5)
Theiler’s murine encephalomyelitis virus
Murine norovirus
Reovirus 3
Mouse rotavirus
Ectromelia virus
Lymphocytic choriomeningitis virus
Polyoma virus
Lactate dehydrogenase-elevating virus
Mouse adenovirus (MAD1, MAD2)
Mouse cytomegalovirus
K virus
Mouse thymic virus
Hantaan virus
Corynebacterium bovis
Corynebacterium spp. (HAC2)

Storage:

Bulk anti-human EGFR In Vivo Antibody – Low Endotoxin (EGFR.1) is stable when stored at 2-8°C. Do not freeze.

Applications:

Flow Cytometry, Immunocytochemistry, Immunofluorescence, IHC (Frozen), Immunoprecipitation, Neutralization, IP-Mass Spectrometry, Western Blot

Application Notes:

Each investigator should determine their own optimal working dilution for specific applications.

Use:

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

Isotype Control:

Mouse IgG2b Isotype Control for In Vivo – Low Endotoxin [MPC-11] (ICH2250)

Related Antibody:

Anti-EGFR In Vivo Antibody – Ultra Low Endotoxin [EGFR.1] (ICH1021UL)

Size:

1mg, 5mg, 25mg, 50mg, 100mg

Endotoxin Level:

Low, Ultra Low

1 review for Bulk anti-Human EGFR antibody (EGFR.1) [ICH1021]

  1. Rated 5 out of 5

    Dr. Shi – June 21, 2021

    Excellent EGFR antibody for use in western blot experiments

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