Anti-Mouse CTLA-4 In Vivo Antibody – Low Endotoxin (UC10-4F10-11) [ICH1085]

ichorbio’s anti-CTLA-4 In Vivo Antibody – Low Endotoxin (UC10-4F10-11) is manufactured in a cGMP compliant, ISO Quality Standard 9001:2015 facility. ichorbio’s low endotoxin antibodies have half the endotoxin of comparable antibodies from our competitors at less than 1.0 EU/mg. If ichorbio’s low endotoxin antibodies are not low enough we also offer ultra low endotoxin antibodies which have even less endotoxin (<0.75EU/mg) at an even higher purity (98% versus 95%). ichorbio: the best antibodies for in vivo research.

CTLA-4

UC10-4F10-11

ichorbio’s UC10-4F10-11 in vivo antibody is available in the following bulk sizes:
1mg, 5mg, 25mg, 50mg and 100mg
ichorbio regularly manufactures multi-gram amounts of our anti-CTLA-4 UC10-4F10-11 clone – please contact us for pricing.

Armenian Hamster IgG1

Ctla4, Cytotoxic T-lymphocyte protein 4, CD152

P09793

Armenian Hamster

Mouse

Anti-CTLA-4 In Vivo Antibody – Low Endotoxin (UC10-4F10-11) recognizes an epitope on Mouse CD152

This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

The UC10-4F10-11 antibody reacts with CD152 (CTLA-4), which is expressed on activated T lymphocytes 2-3 days after stimulation through T cell receptor.

CD152, also known as CTLA-4 or Ly-56 has significant similarity to CD28 in amino acid sequence, structure, and genomic organization. Furthermore, CD152 and CD28 share common B7 family counter-receptors. Unlike CD28, CD152 expression appears to be restricted to activated T cells and CD25+CD4+ regulatory T (Treg) cells. CD152 is thought to play a role in the induction and maintenance of immunological tolerance as well as the development of protective immunity and thymocyte regulation.

Purified Recombinant Mouse CTLA4 Fc Fusion Protein

≥ 2.0 mg/ml

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.

>95% by SDS-PAGE and HPLC

>98% by SDS-PAGE and HPLC

≤ 1.0 EU/mg as determined by the LAL method

≤ 0.75 EU/mg as determined by the LAL method

Aggregation level ≤ 5%

Aggregation level ≤ 1%

This antibody is stable for at least 4 weeks when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 20°C or -80°C. Avoid repeated freeze thaw cycles.

Flow Cytometry, Western Blot, Functional Assays, Immunoprecipitation

We recommend using between 50-250µg per mouse when performing in vivo research using ichorbio’s CTLA-4 low endotoxin antibody clone UC10-4F10-11. This range is based off the most recent publication data using the UC10-4F10-11 clone in vivo.