Bulk anti-CTLA-4 In Vivo Antibody – Low Endotoxin (9H10)
ichorbio’s anti-CTLA-4 In Vivo Antibody – Low Endotoxin (9H10) is manufactured in a cGMP compliant facility. ichorbio’s low endotoxin antibodies have half the endotoxin of comparable antibodies from our competitors at less than 1.0 EU/mg. If ichorbio’s low endotoxin antibodies are not low enough we also offer ultra low endotoxin antibodies which have even less endotoxin (<0.75EU/mg) at an even higher purity (98% versus 95%). ichorbio: the best antibodies for in vivo research.
ichorbio’s 9H10 in vivo antibody is available in the following bulk sizes:
1mg, 5mg, 25mg, 50mg and 100mg
ichorbio regularly manufactures multi-gram amounts of our anti-CTLA-4 9H10 clone – please contact us for pricing.
Syrian Hamster IgG
Ctla4, Cytotoxic T-lymphocyte protein 4, CD152
Anti-CTLA-4 In Vivo Antibody – Low Endotoxin (9H10) recognizes an epitope on Mouse CTLA-4
This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Activated T cells
CTLA4 (Cytotoxic T-Lymphocyte Antigen 4) also known as CD152, is a protein which is expressed on the surface of Helper T cells and plays an important regulatory role in the immune system. CTLA4 is a member of the immunoglobulin superfamily, expressed on the surface of Helper T cells. CTLA4 transmits an inhibitory signal to T cells. CTLA4 is potentially therapeutic in auto-immune diseases4, such as rheumatoid arthritis, HIV, autoimmune thyroid disease, multiple sclerosis and may also be useful during organ transplantation and cancer treatment. Clone 9H10 antibody has been shown to promote T cell co-stimulation by blocking CTLA4 binding, allowing for CD28 binding.
Mouse CTLA-4-human IgG1 fusion protein
1.0 – 5.0 mg/ml
0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.
>95% by SDS-PAGE and HPLC
>98% by SDS-PAGE and HPLC
≤ 1.0 EU/mg as determined by the LAL method
≤ 0.5 EU/mg as determined by the LAL method
Aggregation level ≤ 5%
Aggregation level ≤ 1%
We use the IMPACT test generated by IDEXX Laboratories to guarantee our Ultra Low Endotoxin antibodies are pathogen free. Our hamster antibodies are tested for:
Pneumonia virus of mice
Kilham’s rat virus
Toolan’s H1 virus
Minute virus of mice
Theiler’s murine encephalomyelitis virus
This antibody is stable for at least 4 weeks when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 20°C or -80°C. Avoid repeated freeze thaw cycles.
Western Blot, Blocking, ELISA, Flow Cytometry
We recommend using between 50-250µg per mouse when performing in vivo research using ichorbio’s CTLA-4 low endotoxin antibody clone 9H10. This range is based off the most recent publication data using the 9H10 clone.
Each investigator should determine their own optimal working dilution for specific applications. A number of studies have used 100ug per injection for T-cell modulation in vivo.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.