Anti-Mouse CTLA-4 In Vivo Antibody – Low Endotoxin (9D9) [ICH1096]

ichorbio’s anti-CTLA-4 In Vivo Antibody – Low Endotoxin (9D9) is manufactured in a cGMP compliant, ISO Quality Standard 9001:2015 facility. ichorbio’s low endotoxin antibodies have half the endotoxin of comparable antibodies from our competitors at less than 1.0 EU/mg. If ichorbio’s low endotoxin antibodies are not low enough we also offer ultra low endotoxin antibodies which have even less endotoxin (<0.75EU/mg) at an even higher purity (98% versus 95%). ichorbio: the best antibodies for in vivo research.

CTLA-4

9D9

ichorbio’s 9D9 in vivo antibody is available in the following bulk sizes:
1mg, 5mg, 25mg, 50mg and 100mg
ichorbio regularly manufactures multi-gram amounts of our anti-CTLA-4 9D9 clone – please contact us for pricing.

Mouse IgG2b

Ctla4, Cytotoxic T-lymphocyte protein 4, CD152

P09793

Mouse

Mouse

Anti-CTLA-4 In Vivo Antibody – Low Endotoxin (9D9) recognizes an epitope on Mouse CTLA-4

This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Activated T cells

Negative regulator of T cell activation

Unknown

1.0 – 5.0 mg/ml

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.

>95% by SDS-PAGE and HPLC

>98% by SDS-PAGE and HPLC

≤ 1.0 EU/mg as determined by the LAL method

≤ 0.75 EU/mg as determined by the LAL method

Aggregation level ≤ 5%

Aggregation level ≤ 1%

This antibody is stable for at least 4 weeks when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 20°C or -80°C. Avoid repeated freeze thaw cycles.

Western Blot, Functional Assays

We recommend using between 50-250µg per mouse when performing in vivo research using ichorbio’s CTLA-4 low endotoxin antibody clone 9D9. This range is based off the most recent publication data using the 9D9 clone in vivo.

Each investigator should determine their own optimal working dilution for specific applications.

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

Mouse IgG2b Isotype Control for In Vivo – Low Endotoxin [MPC-11] (ICH2250)

Anti-CTLA-4 In Vivo Antibody – Low Endotoxin [9H10] (ICH1084), Anti-CTLA-4 In Vivo Antibody – Low Endotoxin [UC10-4F10-11] (ICH1085), Anti-CTLA-4 In Vivo Antibody – Ultra Low Endotoxin [9H10] (ICH1084UL)