Anti-Mouse CD8a In Vivo Antibody – Low Endotoxin (53-6.7) [ICH1044]

ichorbio’s anti-CD8a In Vivo Antibody – Low Endotoxin (53-6.7) is manufactured in a cGMP compliant, ISO Quality Standard 9001:2015 facility. ichorbio’s low endotoxin antibodies have half the endotoxin of comparable antibodies from our competitors at less than 1.0 EU/mg. If ichorbio’s low endotoxin antibodies are not low enough we also offer ultra low endotoxin antibodies which have even less endotoxin (<0.75EU/mg) at an even higher purity (98% versus 95%). ichorbio: the best antibodies for in vivo research.

CD8a

53-6.7

ichorbio’s 53-6.7 in vivo antibody is available in the following bulk sizes:
1mg, 5mg, 25mg, 50mg and 100mg
ichorbio regularly manufactures multi-gram amounts of our anti-CD8a 53-6.7 clone – please contact us for pricing.

Rat IgG2a

CD8 alpha chain, Lyt-2, Lyt2

P01731

Rat

Mouse

Anti-CD8a In Vivo Antibody – Low Endotoxin (53-6.7) recognizes Lyt 2, lymphocyte differentiation antigen. The antibody precipitates 44 kDa and 33 kDa proteins and reacts with non-polymorphic determinant.

Present on the surface of most thymocytes and a subpopulation of mature T-lymphocytes which include most T suppressor/cytotoxic cells. CD8 participates in T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase lck.

CD8, a.k.a Lyt-2, Ly-2, or T8, consists of disulfide-linked A and β chains that form the A(CD8a)/β(CD8b) heterodimer and A/A homodimer. CD8a is a 34 kD protein that belongs to the immunoglobulin family. The CD8 A/β heterodimer is expressed on the surface of most thymocytes and a subset of mature TCR A/β T cells.

Mouse thymus or spleen

1.0 – 5.0 mg/ml

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.

>95% by SDS-PAGE and HPLC

>98% by SDS-PAGE and HPLC

≤ 1.0 EU/mg as determined by the LAL method

≤ 0.75 EU/mg as determined by the LAL method

Aggregation level ≤ 5%

Aggregation level ≤ 1%

This antibody is stable for at least 4 weeks when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 20°C or -80°C. Avoid repeated freeze thaw cycles.

Immunoprecipitation, Western Blot, Flow Cytometry, CyTOF, IHC (Frozen)

Immunoprecipitation: Rat Anti-Mouse CD8a (Clone 53-6.7) precipitates 44 kDa and 33 kDa proteins.
Each investigator should determine their own optimal working dilution for specific applications.

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

Rat IgG2a In Vivo Isotype Control – Low Endotoxin [1-1] (ICH2244)

Anti-CD8a In Vivo Antibody – Low Endotoxin [2.43] (ICH1045), Anti-CD8a In Vivo Antibody – Ultra Low Endotoxin [2.43] (ICH1045UL)