Anti-CD86 In Vivo Antibody – Low Endotoxin (GL1) [ICH1038]

ichorbio’s anti-CD86 In Vivo Antibody – Low Endotoxin (GL1) is manufactured in a cGMP compliant, ISO Quality Standard 9001:2015 facility. ichorbio’s low endotoxin antibodies have half the endotoxin of comparable antibodies from our competitors at less than 1.0 EU/mg. If ichorbio’s low endotoxin antibodies are not low enough we also offer ultra low endotoxin antibodies which have even less endotoxin (<0.75EU/mg) at an even higher purity (98% versus 95%). ichorbio: the best antibodies for in vivo research.

CD86

GL1

ichorbio’s GL1 in vivo antibody is available in the following bulk sizes:
1mg, 5mg, 25mg, 50mg and 100mg
ichorbio regularly manufactures multi-gram amounts of our anti-CD86 GL1 clone – please contact us for pricing.

Rat IgG2a

Activation B7-2 antigen, Early T-cell costimulatory molecule 1, ETC-1

P42082

Rat

Mouse

Anti-CD86 In Vivo Antibody – Low Endotoxin (GL1) recognizes an epitope on Mouse CD86

This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

B cells and T cells (upregulated upon activation), macrophages, dendritic cells, and astrocytes

CD86, also known as B7-2, is a type I transmembrane glycoprotein and a member of the Ig gene superfamily. CD86 is a ligand for CD28 and CD152 (CTLA-4). CD86 is related to the CD80 molecule and functions as a counterreceptor for CD28 and CTLA-4. The GL1 clone has been shown to provide critical signals for T-Cell-dependent responses in vivo and in vitro.

LPS-activated CBA/Ca mouse splenic B cells

1.0 mg/ml

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.

>95% by SDS-PAGE and HPLC

>98% by SDS-PAGE and HPLC

≤ 1.0 EU/mg as determined by the LAL method

≤ 0.75 EU/mg as determined by the LAL method

Aggregation level ≤ 5%

Aggregation level ≤ 1%

This antibody is stable for at least 4 weeks when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 20°C or -80°C. Avoid repeated freeze thaw cycles.

Flow Cytometry, Immunoprecipitation, IHC (Frozen), Immunocytochemistry, Blocking, Immunofluorescence, Western Blot, CyTOF

Each investigator should determine their own optimal working dilution for specific applications.

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

Rat IgG2a In Vivo Isotype Control – Low Endotoxin [1-1] (ICH2244)