Bulk anti-Human CD8 antibody (UCHT-4) [ICH1005]

ichorbio’s bulk anti-human CD8 antibody (UCHT-4) is manufactured in a cGMP compliant, ISO Quality Standard 9001:2015 facility. ichorbio’s low endotoxin antibodies have half the endotoxin of comparable antibodies from our competitors at less than 1.0 EU/mg. If ichorbio’s low endotoxin antibodies are not low enough we also offer ultra low endotoxin antibodies which have even less endotoxin (<0.75EU/mg) at an even higher purity (98% versus 95%). ichorbio: the best antibodies for in vivo research.

CD8

UCHT-4

Mouse IgG2a

Mouse

Human

Anti-human CD8 antibody (UCHT-4) recognizes Human CD8. Anti-Human CD8 recognizes a T-cell glycoprotein cell surface complex (Mr 32 kDa) which defines the cytotoxic/suppressor population of mature T-lymphocytes. The CD8 complex interacts with class I MHC molecules resulting in increased adhesion between CD8+ T-lymphocytes and their target cells. Binding of the CD8 antigen to Class I MHC molecules enhances T-cell activation. The cytoplasmic portion of the CD8 antigen is associated with a protein tyrosine kinase (p56lck).

This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

The CD8 antigen is expressed on approximately 19-48% of normal human peripheral blood lymphocytes and > 90% of normal thymocytes. It is also present on a subset of NK cells. Subjects infected with HIV have been shown to exhibit increased levels of CD8+ lymphocytes with a continual decrease in CD4+ lymphocytes.

Anti-CD8 may be used in studies of immunoregulation and T-lymphocyte mediated suppression, autoimmune disorders, immunodeficiency states and acute lymphoblastic leukemia and lymphoma.

1.0 – 5.0 mg/ml

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.

>95% by SDS-PAGE and HPLC

>98% by SDS-PAGE and HPLC

<1.0 EU/mg as determined by the LAL method

≤ 0.75 EU/mg as determined by the LAL method

Aggregation level ≤ 5%

Aggregation level ≤ 1%

This antibody is stable for at least 4 weeks when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 20°C or -80°C. Avoid repeated freeze thaw cycles.

Flow Cytometry, Western Blot, Immunoprecipitation, Functional Assays, IHC, Immunofluorescence, ELISA

Each investigator should determine their own optimal working dilution for specific applications.

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

Mouse IgG2a In Vivo Isotype Control – Low Endotoxin [C1.18.4] (ICH2245)

Anti-CD8 In Vivo Antibody – Low Endotoxin [YTS-169] (ICH1043), Anti-CD8 In Vivo Antibody – Ultra Low Endotoxin [YTS-169] (ICH1043UL)