Anti-CD3e In Vivo Antibody - Low Endotoxin (145-2C11)

ICH1041 is up to 19% cheaper for academia & non-profits and up to 52% cheaper for industry than the equivalent products BE0001-1 (low endotoxin) and BP0001-1 (ultra-low endotoxin) from Bio X Cell. Product Benefits:

ichorbio's anti-CD3e In Vivo Antibody - Low Endotoxin (145-2C11) is manufactured in a cGMP compliant facility. ichorbio's low endotoxin antibodies have half the endotoxin of comparable antibodies from our competitors at less than 1.0 EU/mg. If ichorbio's low endotoxin antibodies are not low enough we also offer ultra low endotoxin antibodies which have even less endotoxin (<0.75EU/mg) at an even higher purity (98% versus 95%). ichorbio: the best antibodies for in vivo research.

Target:

CD3e

Clone:

145-2C11

Size:

ichorbio's 145-2C11 in vivo antibody is available in the following bulk sizes: 1mg, 5mg, 25mg, 50mg and 100mg ichorbio regularly manufactures multi-gram amounts of our anti-CD3e 145-2C11 clone - please contact us for pricing.

Isotype:

Armenian Hamster IgG

Other Names:

T3/Leu-4 epsilon chain, CD3 epsilon chain, CD3e

Host:

Armenian Hamster

Species Reactivity:

Mouse

Specificity:

Anti-CD3e In Vivo Antibody - Low Endotoxin (145-2C11) recognizes an epitope on Mouse CD3e

Purification Method:

This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Antigen Distribution:

The CD3 complex is present on thymocytes (differentiation dependent), mature T cells, NK-T cells of all mouse strains tested.

Background:

CD3e is a 20 kD transmembrane protein and is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex.

Immunogen:

H-2Kb-specific mouse cytotoxic T lymphocyte clone BM10-37

Concentration:

1.0 mg/ml

Formulation:

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.

Purity:

>95% by SDS-PAGE and HPLC

>98% by SDS-PAGE and HPLC

Endotoxin:

≤ 1.0 EU/mg as determined by the LAL method

≤ 0.75 EU/mg as determined by the LAL method

Aggregation:

Aggregation level ≤ 5%

Aggregation level ≤ 1%

IMPACT Pathogen Test:

We use the IMPACT test generated by IDEXX Laboratories to guarantee our Ultra Low Endotoxin antibodies are pathogen free. Our hamster antibodies are tested for: Mycoplasma spp Mycoplasma pulmonis Pneumonia virus of mice Kilham's rat virus Toolan's H1 virus Hamster parvovirus Lymphocytic choriomeningitis Minute virus of mice Theiler's murine encephalomyelitis virus Sendai virus Reovirus 3 Hantaan virus

Storage:

This antibody is stable for at least 4 weeks when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 20°C or -80°C. Avoid repeated freeze thaw cycles.

Applications:

Immunoprecipitation, Blocking

Application Notes:

Blocking: The 145-2C11 antibody has been reported to block the binding of 17A2 antibody to CD3 epsilon-specific T cells Each investigator should determine their own optimal working dilution for specific applications.

Use:

Products are for research use only.

Isotype Control:

Armenian Hamster IgG Isotype Control for In Vivo - Low Endotoxin [PIP] (ICH2251)

Antibodies against the same target:

Anti-CD3e In Vivo Antibody - Low Endotoxin [500A2] (ICH1040), Anti-CD3e In Vivo Antibody - Ultra Low Endotoxin [500A2] (ICH1040UL)