Anti-CD3e In Vivo Antibody – Low Endotoxin (145-2C11) [ICH1041]

ichorbio’s anti-CD3e In Vivo Antibody – Low Endotoxin (145-2C11) is manufactured in a cGMP compliant facility. ichorbio’s low endotoxin antibodies have half the endotoxin of comparable antibodies from our competitors at less than 1.0 EU/mg. If ichorbio’s low endotoxin antibodies are not low enough we also offer ultra low endotoxin antibodies which have even less endotoxin (<0.75EU/mg) at an even higher purity (98% versus 95%). ichorbio: the best antibodies for in vivo research.

CD3e

145-2C11

ichorbio’s 145-2C11 in vivo antibody is available in the following bulk sizes:
1mg, 5mg, 25mg, 50mg and 100mg
ichorbio regularly manufactures multi-gram amounts of our anti-CD3e 145-2C11 clone – please contact us for pricing.

Armenian Hamster IgG

T3/Leu-4 epsilon chain, CD3 epsilon chain, CD3e

Armenian Hamster

Mouse

Anti-CD3e In Vivo Antibody – Low Endotoxin (145-2C11) recognizes an epitope on Mouse CD3e

This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

The CD3 complex is present on thymocytes (differentiation dependent), mature T cells, NK-T cells of all mouse strains tested.

CD3e is a 20 kD transmembrane protein and is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex.

H-2Kb-specific mouse cytotoxic T lymphocyte clone BM10-37

1.0 mg/ml

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.

>95% by SDS-PAGE and HPLC

>98% by SDS-PAGE and HPLC

≤ 1.0 EU/mg as determined by the LAL method

≤ 0.75 EU/mg as determined by the LAL method

Aggregation level ≤ 5%

Aggregation level ≤ 1%

This antibody is stable for at least 4 weeks when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 20°C or -80°C. Avoid repeated freeze thaw cycles.

Immunoprecipitation, Blocking

Blocking: The 145-2C11 antibody has been reported to block the binding of 17A2 antibody to CD3 epsilon-specific T cells
Each investigator should determine their own optimal working dilution for specific applications.

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

Armenian Hamster IgG Isotype Control for In Vivo – Low Endotoxin [PIP] (ICH2251)

Anti-CD3e In Vivo Antibody – Low Endotoxin [500A2] (ICH1040), Anti-CD3e In Vivo Antibody – Ultra Low Endotoxin [500A2] (ICH1040UL)