Bulk anti-CD32/CD16 In Vivo Antibody – Low Endotoxin (2.4G2)
ICH1056 is up to 11% cheaper for academia & non-profits and up to 51% cheaper for industry than the equivalent products BE0307 (low endotoxin) from Bio X Cell.
We guarantee that our antibody will perform exactly the same in your experiments as BE0307 from Bio X Cell: if it doesn’t we will refund your purchase and pay for the Bio X Cell antibody to be delivered to you as soon as possible.
ichorbio’s anti-CD32/CD16 In Vivo Antibody – Low Endotoxin (2.4G2) is manufactured in a cGMP compliant, ISO Quality Standard 9001:2015 facility. ichorbio’s low endotoxin antibodies have half the endotoxin of comparable antibodies from our competitors at less than 1.0 EU/mg. If ichorbio’s low endotoxin antibodies are not low enough we also offer ultra low endotoxin antibodies which have even less endotoxin (<0.75EU/mg) at an even higher purity (98% versus 95%). ichorbio: the best antibodies for in vivo research.
ichorbio’s 2.4G2 in vivo antibody is available in the following bulk sizes:
1mg, 5mg, 25mg, 50mg and 100mg
ichorbio regularly manufactures multi-gram amounts of our anti-CD32/CD16 2.4G2 clone – please contact us for pricing.
Low affinity immunoglobulin gamma Fc region receptor II, Fcgr2, Fc-gamma-RIIB, FcRII, IgG Fc receptor II beta, Ly-17, Low affinity immunoglobulin gamma Fc region receptor III, Fcgr3, FcRIII, Fc-gamma RIII
Anti-CD32/CD16 In Vivo Antibody – Low Endotoxin (2.4G2) recognizes the FcγII and FcγIII receptors
This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
The Fc gamma II/III receptors are present on mouse natural killer cells, monocytes, macrophages, granulocytes, B-cells and most fetal thymocytes.
CD16 is a low-affinity IgG Fc receptor III and CD32 is FcR II. CD16/CD32 are expressed on B cells, monocytes/macrophages, NK cells, granulocytes, mast cells, and dendritic cells. The Fc receptors bind antibody-antigen immune complexes and mediate adaptive immune responses. Clone 2.4G2 antibody is used in flow cytometry staining experiments to prevent non-specific binding of IgG to the FcγIII and FcγII, and possibly FcγI, receptors prior to staining with antigen-specific primary antibodies. The Fab fragments of the 2.4G2 antibody have also been used to block Fc receptors in vivo.
0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.
>95% by SDS-PAGE and HPLC
>98% by SDS-PAGE and HPLC
≤ 1.0 EU/mg as determined by the LAL method
≤ 0.75 EU/mg as determined by the LAL method
Aggregation level ≤ 5%
Aggregation level ≤ 1%
anti-CD32/CD16 In Vivo Antibody – Low Endotoxin (2.4G2) is stable for at least four (4) weeks when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
Western Blot, Blocking, Flow cytometry, IHC (Frozen), Immunoprecipitation, CODEX
Each investigator should determine their own optimal working dilution for specific applications.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.