Anti-Mouse CD3 In Vivo Antibody – Low Endotoxin (17A2) [ICH1039]

ichorbio’s anti-CD3 In Vivo Antibody – Low Endotoxin (17A2) is manufactured in a cGMP compliant, ISO Quality Standard 9001:2015 facility. ichorbio’s low endotoxin antibodies have half the endotoxin of comparable antibodies from our competitors at less than 1.0 EU/mg. If ichorbio’s low endotoxin antibodies are not low enough we also offer ultra low endotoxin antibodies which have even less endotoxin (<0.75EU/mg) at an even higher purity (98% versus 95%). ichorbio: the best antibodies for in vivo research.

CD3

17A2

ichorbio’s 17A2 in vivo antibody is available in the following bulk sizes:
1mg, 5mg, 25mg, 50mg and 100mg
ichorbio regularly manufactures multi-gram amounts of our anti-CD3 17A2 clone – please contact us for pricing.

Rat IgG2b

T3/Leu-4 epsilon chain, CD3 epsilon chain, CD3e, Cd3g, T3 gamma chain, CD3 gamma chain

Rat

Mouse

Anti-CD3 In Vivo Antibody – Low Endotoxin (17A2) recognizes an epitope on Mouse CD3. The 17A2 clone reacts with the epsilon and gamma chains and not delta nor zeta of CD3.

This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

The CD3 complex is present on thymocytes and mature T-lymphocytes of all mouse strains tested.

CD3, is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 is composed of CD3ε, δ, γ and ζ chains. It forms a TCR complex by associating with TCR A/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex.

gamma/delta TCR-positive T-T hybridoma D1

1.0 mg/ml

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.

>95% by SDS-PAGE and HPLC

>98% by SDS-PAGE and HPLC

≤ 1.0 EU/mg as determined by the LAL method

≤ 0.75 EU/mg as determined by the LAL method

Aggregation level ≤ 5%

Aggregation level ≤ 1%

anti-CD3 In Vivo Antibody – Low Endotoxin (17A2) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

Immunoprecipitation, Western Blot, Flow Cytometry, IHC (Frozen), Immunocytochemistry

Each investigator should determine their own optimal working dilution for specific applications.

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

Rat IgG2b In Vivo Isotype Control – Low Endotoxin [1-2] (ICH2243)

Anti-CD3 In Vivo Antibody – Low Endotoxin [UCHT-1] (ICH1002), Anti-CD3 In Vivo Antibody – Low Endotoxin [HIT3a] (ICH1003)