Bulk anti-CD278 In Vivo Antibody – Low Endotoxin (7E.17G9)
ichorbio’s anti-CD278 In Vivo Antibody – Low Endotoxin (7E.17G9) is manufactured in a cGMP compliant, ISO Quality Standard 9001:2015 facility. ichorbio’s low endotoxin antibodies have half the endotoxin of comparable antibodies from our competitors at less than 1.0 EU/mg. If ichorbio’s low endotoxin antibodies are not low enough we also offer ultra low endotoxin antibodies which have even less endotoxin (<0.75EU/mg) at an even higher purity (98% versus 95%). ichorbio: the best antibodies for in vivo research.
Inducible T-cell costimulator, Icos, Activation-inducible lymphocyte immunomediatory molecule, CD28 and CTLA-4-like protein, CCLP, CD28-related protein 1, CRP-1, Ailim
Anti-CD278 In Vivo Antibody – Low Endotoxin (7E.17G9) recognizes an epitope on Mouse ICOS
This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Inducible co-stimulator (ICOS), also called ALIM and H4, is a homodimeric protein and member of the CD28 family of immune co-stimulatory receptors. Other family members are CD28, CTLA-4 and PD-1. ICOS enhances all basic T cell responses to a foreign antigen, namely proliferation, secretion of lymphokines, up-regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B cells. Unlike the constitutively expressed CD28, ICOS has to be de novo induced on the T cell surface, does not up-regulate the production of IL-2, but superinduces the synthesis of IL-10, a B cell-differentiation factor. In vivo, ICOS is highly expressed on tonsillar T cells, which are closely associated with B cells in the apical light zone of germinal centres, the site of terminal B cell maturation. ICOS regulates CD28-dependent and CD28-independent CD4 (+) subset (Th1 and Th2) responses. It is a major regulator of the adaptive immune system and therefore, has a protective role in inflammatory autoimmune diseases.
1.0 – 5.0 mg/ml
0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.
>95% by SDS-PAGE and HPLC
>98% by SDS-PAGE and HPLC
≤ 1.0 EU/mg as determined by the LAL method
≤ 0.75 EU/mg as determined by the LAL method
Aggregation level ≤ 5%
Aggregation level ≤ 1%
We use the IMPACT test generated by IDEXX Laboratories to guarantee our Ultra Low Endotoxin antibodies are pathogen free. Our rat antibodies are tested for:
Pneumonia virus of mice
Kilham’s rat virus
Toolan’s H1 virus
Lymphocytic choriomeningitis virus
Rat minute virus
This antibody is stable for at least 4 weeks when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 20°C or -80°C. Avoid repeated freeze thaw cycles.
Western Blot, Blocking, Functional Assays, Flow Cytometry
Each investigator should determine their own optimal working dilution for specific applications.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.