Bulk anti-CD209b In Vivo Antibody – Low Endotoxin (22D1)
ichorbio’s anti-CD209b In Vivo Antibody – Low Endotoxin (22D1) is manufactured in a cGMP compliant, ISO Quality Standard 9001:2015 facility. ichorbio’s low endotoxin antibodies have half the endotoxin of comparable antibodies from our competitors at less than 1.0 EU/mg. If ichorbio’s low endotoxin antibodies are not low enough we also offer ultra low endotoxin antibodies which have even less endotoxin (<0.75EU/mg) at an even higher purity (98% versus 95%). ichorbio: the best antibodies for in vivo research.
ichorbio’s 22D1 in vivo antibody is available in the following bulk sizes:
1mg, 5mg, 25mg, 50mg and 100mg
ichorbio regularly manufactures multi-gram amounts of our anti-CD209b 22D1 clone – please contact us for pricing.
Armenian Hamster IgG1
DC-SIGN-related protein 1, DC-SIGNR1, OtB7
Anti-CD209b In Vivo Antibody – Low Endotoxin (22D1) recognizes an epitope on Mouse CD209b, also known as SIGN-R1
This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Purified Recombinant Mouse CD209b (C-terminal peptide)
≥ 2.0 mg/ml
0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.
>95% by SDS-PAGE and HPLC
>98% by SDS-PAGE and HPLC
≤ 1.0 EU/mg as determined by the LAL method
≤ 0.75 EU/mg as determined by the LAL method
Aggregation level ≤ 5%
Aggregation level ≤ 1%
This antibody is stable for at least 4 weeks when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 20°C or -80°C. Avoid repeated freeze thaw cycles.
IHC (Frozen), Flow Cytometry, Western Blot, Blocking, Immunofluorescence, ELISA, Immunocytochemistry
Blocking: Clone 22D1 has been reported to block CD209b in vivo.
Each investigator should determine their own optimal working dilution for specific applications.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
Immunofluorescence analysis of paraffin-embedded breast cancer tissue section labeling CD209b (1:100) overnight at 4˚, followed by goat anti-mouse IgG H&L (Alexa Fluor ® 647-red) secondary antibody (1:500 dilution). MDA-MB-231 cells were injected via mouse nipple and grown for 5 weeks. The nuclear counter stain is DAPI (blue). Image was acquired on a Nikon A1R microscope system at 4x magnification (first image) or 60x magnification (second image).