Bulk anti-human CD20 antibody (2H7) [ICH1012]

ICH1012 is up to 11% cheaper for academia & non-profits and up to 51% cheaper for industry than the equivalent products BE0276 (low endotoxin) from Bio X Cell. Product Benefits:

ichorbio's bulk anti-human CD20 antibody (2H7) is manufactured in a cGMP compliant facility. ichorbio's low endotoxin antibodies have half the endotoxin of comparable antibodies from our competitors at less than 1.0 EU/mg. If ichorbio's low endotoxin antibodies are not low enough we also offer ultra low endotoxin antibodies which have even less endotoxin (<0.75EU/mg) at an even higher purity (98% versus 95%). ichorbio: the best antibodies for in vivo research.

Target:

CD20

Clone:

2H7

Size:

ichorbio's 2H7 in vivo antibody is available in the following bulk sizes: 1mg, 5mg, 25mg, 50mg and 100mg ichorbio regularly manufactures multi-gram amounts of our anti-CD20 2H7 clone - please contact us for pricing.

Isotype:

Mouse IgG2b

Other Names:

B-lymphocyte surface antigen B1, Bp35, Leukocyte surface antigen Leu-16, Membrane-spanning 4-domains subfamily A member 1, MS4A1

Uniprot:

P11836

Host:

Mouse

Species Reactivity:

Human

Specificity:

Bulk anti-human CD20 antibody (2H7) recognizes an epitope on Human CD20

Purification Method:

This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Background:

The CD20 antigen, also called MS4A1, B1 and Bp35, is a non-glycosylated, transmembrane spanning, phosphoprotein. The CD20 protein is present on human pre B lymphocytes and on B lymphocytes at all stages of maturation, except on plasma cells, and is synchronous with the expression of surface IgM. It is also expressed in some follicular dendritic cells and at low levels on a T cell subset. CD20 has been shown to be heavily phosphorylated in activated B cells and malignant B cells. Homo-oligomeric complexes of CD20 are thought to form Ca2+ conductive ion channels in the plasma membrane of B cells. The CD20 molecule is involved in B cell activation and is associated with various Src family kinases (Lyn, Lck, Fyn), and exists in a complex with MHC class I and II, CD53, CD81, and CD82. Extensive CD20 cross-linking may also influence cell death by apoptosis. Therapy with antibodies against CD20 is a widely used regimen in the treatment of all B cell lymphomas and leukemias.

Concentration:

1.0 - 5.0 mg/ml

Formulation:

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.

Purity:

>95% by SDS-PAGE and HPLC

>98% by SDS-PAGE and HPLC

Endotoxin:

≤ 1.0 EU/mg as determined by the LAL method

≤ 0.75 EU/mg as determined by the LAL method

Aggregation:

Aggregation level ≤ 5%

Aggregation level ≤ 1%

IMPACT Pathogen Test:

We use the IMPACT test generated by IDEXX Laboratories to guarantee our Ultra Low Endotoxin antibodies are pathogen free. Our mouse antibodies are tested for: Mycoplasma spp. Mycoplasma pulmonis Sendai virus Mouse hepatitis virus Pneumonia virus of mice Minute virus of mice Mouse parvovirus (MPV1-5) Theiler's murine encephalomyelitis virus Murine norovirus Reovirus 3 Mouse rotavirus Ectromelia virus Lymphocytic choriomeningitis virus Polyoma virus Lactate dehydrogenase-elevating virus Mouse adenovirus (MAD1, MAD2) Mouse cytomegalovirus K virus Mouse thymic virus Hantaan virus Corynebacterium bovis Corynebacterium spp. (HAC2)

Storage:

This antibody is stable for at least 4 weeks when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 20°C or -80°C. Avoid repeated freeze thaw cycles.

Applications:

Flow Cytometry, CyTOF, IHC (Frozen), Immunoprecipitation

Application Notes:

Each investigator should determine their own optimal working dilution for specific applications.

Use:

Products are for research use only.

Isotype Control:

Mouse IgG2b Isotype Control for In Vivo - Low Endotoxin [MPC-11] (ICH2250)