Bulk anti-CD2 In Vivo Antibody – Low Endotoxin (G11)
ichorbio’s anti-CD2 In Vivo Antibody – Low Endotoxin (G11) is manufactured in a cGMP compliant, ISO Quality Standard 9001:2015 facility. ichorbio’s low endotoxin antibodies have half the endotoxin of comparable antibodies from our competitors at less than 1.0 EU/mg. If ichorbio’s low endotoxin antibodies are not low enough we also offer ultra low endotoxin antibodies which have even less endotoxin (<0.75EU/mg) at an even higher purity (98% versus 95%). ichorbio: the best antibodies for in vivo research.
Erythrocyte receptor, LFA-2, LFA-3 receptor, Rosette receptor, T-cell surface antigen T11/Leu-5
Anti-CD2 In Vivo Antibody – Low Endotoxin (G11) recognizes Human CD2. Anti-Human CD2 recognizes a cell surface glycoprotein (Mr 45-59 kDa) on human lymphocytes
This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
The CD2 antigen is present on approximately 80% of normal peripheral blood lymphocytes, >95% of thymocytes, 100% of mature circulating T-cells and a subset of NK cells.
Appropriate pairs of anti-CD2 antibodies will stimulate peripheral T-cell proliferation and effector function. The CD2 molecule has been shown to be the receptor for leukocyte function antigen (LFA-3/CD58), the binding of which augments T-cell activation mediated by the T-cell antigen receptor (TCR). Anti-Human CD2 can be used to deplete CD2 positive cells by complement-mediated cytotoxicity. Analyses of NK lymphocyte subsets and identification of lymphomas and leukemias of T-cell origin have been done using Anti-CD2 antibodies.
1.0 – 5.0 mg/ml
0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.
>95% by SDS-PAGE and HPLC
>98% by SDS-PAGE and HPLC
≤ 1.0 EU/mg as determined by the LAL method
≤ 0.75 EU/mg as determined by the LAL method
Aggregation level ≤ 5%
Aggregation level ≤ 1%
anti-CD2 In Vivo Antibody – Low Endotoxin (G11) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
Western Blot, Functional Assays
Each investigator should determine their own optimal working dilution for specific applications.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.