Bulk anti-Human CD2 antibody (G11) [ICH1001]

ichorbio’s anti-Human CD2 antibody (G11) is manufactured in a cGMP compliant, ISO Quality Standard 9001:2015 facility. ichorbio’s low endotoxin antibodies have half the endotoxin of comparable antibodies from our competitors at less than 1.0 EU/mg. If ichorbio’s low endotoxin antibodies are not low enough we also offer ultra low endotoxin antibodies which have even less endotoxin (<0.75EU/mg) at an even higher purity (98% versus 95%). ichorbio: the best antibodies for in vivo research.

CD2

G11

Mouse IgG2a

Erythrocyte receptor, LFA-2, LFA-3 receptor, Rosette receptor, T-cell surface antigen T11/Leu-5

P06729

Mouse

Human

Anti-Human CD2 antibody (G11) recognizes Human CD2. Anti-Human CD2 recognizes a cell surface glycoprotein (Mr 45-59 kDa) on human lymphocytes

This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

The CD2 antigen is present on approximately 80% of normal peripheral blood lymphocytes, >95% of thymocytes, 100% of mature circulating T-cells and a subset of NK cells.

Appropriate pairs of anti-CD2 antibodies will stimulate peripheral T-cell proliferation and effector function. The CD2 molecule has been shown to be the receptor for leukocyte function antigen (LFA-3/CD58), the binding of which augments T-cell activation mediated by the T-cell antigen receptor (TCR). Anti-Human CD2 can be used to deplete CD2 positive cells by complement-mediated cytotoxicity. Analyses of NK lymphocyte subsets and identification of lymphomas and leukemias of T-cell origin have been done using Anti-CD2 antibodies.

1.0 – 5.0 mg/ml

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.

>95% by SDS-PAGE and HPLC

>98% by SDS-PAGE and HPLC

≤ 1.0 EU/mg as determined by the LAL method

≤ 0.75 EU/mg as determined by the LAL method

Aggregation level ≤ 5%

Aggregation level ≤ 1%

This antibody is stable for at least 4 weeks when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 20°C or -80°C. Avoid repeated freeze thaw cycles.

Western Blot, Functional Assays

Each investigator should determine their own optimal working dilution for specific applications.

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

Mouse IgG2a In Vivo Isotype Control – Low Endotoxin [C1.18.4] (ICH2245)