Bulk anti-Human CD119 antibody (GIR 208) [ICH1028]

ichorbio’s bulk anti-human CD119 antibody (GIR 208) is manufactured in a cGMP compliant facility. ichorbio’s low endotoxin antibodies have half the endotoxin of comparable antibodies from our competitors at less than 1.0 EU/mg. If ichorbio’s low endotoxin antibodies are not low enough we also offer ultra low endotoxin antibodies which have even less endotoxin (<0.75EU/mg) at an even higher purity (98% versus 95%). ichorbio: the best antibodies for in vivo research.

CD119

GIR 208

Mouse IgG1

Interferon gamma receptor 1, IFN-gamma receptor 1, IFN-gamma-R1, CDw119, Interferon gamma receptor alpha-chain2, IFN-gamma-R-alpha

P15260

Mouse

Human

Bulk anti-human CD119 antibody (GIR 208) recognizes Human IFN gamma R alpha

This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

The IFN-gamma receptor is expressed at moderate levels on virtually every cell with the exception of erythrocytes.

Interferon-gamma (IFN-gamma) exerts its biologic effects by interacting with a single high affinity receptor, IFN-gamma receptor. This receptor is present on almost all cell types except mature erythrocytes. The IFN-gamma receptor consists of two polypeptide chains alpha and beta and requires association of JAK1, JAK2 and Stat1 for IFN-gamma signal transduction. In signal transduction, IFN-gamma induces tyrosine phosphorylation of IFN-gamma receptor alpha (IFNGR1) leading to the formation of a docking site on the activated receptor for Stat1, which specifically activates IFN-gamma induced gene transcription. The IFN-gamma receptor is structurally related to the recently cloned IL-10 receptor. GIR 208 binds IFNGR1 and blocks binding of IFN-gamma to the receptor.

Human IFN-gamma R alpha, Purified from human placenta

1.0 – 5.0 mg/ml

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.

>95% by SDS-PAGE and HPLC

>98% by SDS-PAGE and HPLC

≤ 1.0 EU/mg as determined by the LAL method

≤ 0.75 EU/mg as determined by the LAL method

Aggregation level ≤ 5%

Aggregation level ≤ 1%

This antibody is stable for at least 4 weeks when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 20°C or -80°C. Avoid repeated freeze thaw cycles.

Immunoprecipitation, Western Blot, ELISA, Neutralization, Flow Cytometry

Each investigator should determine their own optimal working dilution for specific applications.

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

Mouse IgG1 Isotype Control for In Vivo – Low Endotoxin [HKSP] (ICH2247)