Anti-Mouse 4-1BB In Vivo Antibody – Low Endotoxin (3H3) [ICH1036]

ichorbio’s anti-4-1BB In Vivo Antibody – Low Endotoxin (3H3) is manufactured in a cGMP compliant, ISO Quality Standard 9001:2015 facility. ichorbio’s low endotoxin antibodies have half the endotoxin of comparable antibodies from our competitors at less than 1.0 EU/mg. If ichorbio’s low endotoxin antibodies are not low enough we also offer ultra low endotoxin antibodies which have even less endotoxin (<0.75EU/mg) at an even higher purity (98% versus 95%). ichorbio: the best antibodies for in vivo research.

4-1BB

3H3

ichorbio’s 3H3 in vivo antibody is available in the following bulk sizes:
1mg, 5mg, 25mg, 50mg and 100mg
ichorbio regularly manufactures multi-gram amounts of our anti-4-1BB 3H3 clone – please contact us for pricing.

Rat IgG2a

4-1BB ligand receptor, T-cell antigen 4-1BB, Tnfrsf9, Ila, Ly63, CD137

P20334

Rat

Mouse, Rat

Anti-4-1BB In Vivo Antibody – Low Endotoxin (3H3) recognizes an epitope on Mouse 4-1BB

This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Activated T cells and B cells

4-1BB (CD137) is a costimulatory molecule expressed on the surface of activated T cells and plays a role in T-cell-mediated proliferative response. 4-1BB signal regulates CD28 co-stimulation in the targeted subset cells to favor Th1 development and maintain long-term cell growth. 4-1BB is also expressed on dendritic cells (DCs) and controls the duration, DC-T interaction, and, therefore, immunogenicity. Clone 3H3 antibody is an agonistic antibody that has been shown to stimulate 4-1BB signaling in vivo.

Unknown

1.0 – 5.0 mg/ml

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.

>95% by SDS-PAGE and HPLC

>98% by SDS-PAGE and HPLC

≤ 1.0 EU/mg as determined by the LAL method

<0.5 EU/mg as determined by the LAL method

Aggregation level ≤ 5%

Aggregation level ≤ 1%

anti-4-1BB In Vivo Antibody – Low Endotoxin (3H3) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

Western Blot, Functional Assays

Each investigator should determine their own optimal working dilution for specific applications.

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

Rat IgG2a In Vivo Isotype Control – Low Endotoxin [1-1] (ICH2244)

Anti-4-1BB In Vivo Antibody – Ultra Low Endotoxin [3H3] (ICH1036UL)