Bulk anti-TNF alpha In Vivo Antibody – Low Endotoxin (TN3-19.12)
ichorbio’s anti-TNF alpha In Vivo Antibody – Low Endotoxin (TN3-19.12) is manufactured in a cGMP compliant facility. ichorbio’s low endotoxin antibodies have half the endotoxin of comparable antibodies from our competitors at less than 1.0 EU/mg. If ichorbio’s low endotoxin antibodies are not low enough we also offer ultra low endotoxin antibodies which have even less endotoxin (<0.75EU/mg) at an even higher purity (98% versus 95%). ichorbio: the best antibodies for in vivo research.
ichorbio’s TN3-19.12 in vivo antibody is available in the following bulk sizes:
1mg, 5mg, 25mg, 50mg and 100mg
ichorbio regularly manufactures multi-gram amounts of our anti-TNF alpha TN3-19.12 clone – please contact us for pricing.
Armenian Hamster IgG
Cachectin, TNFa, Tumor necrosis factor ligand superfamily member 2, Tnfsf2
Anti-TNF alpha In Vivo Antibody – Low Endotoxin (TN3-19.12) recognizes Mouse TNFa
This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Activated monocytes, neutrophils, macrophages, T cells, B cells, NK cells, LAK cells
The tumor necrosis factor (TNF-alpha) is a multifaceted polypeptide cytokine known as a mediator of inflammation and immunity. It may mediate some of the significant changes in cellular homeostasis which accompany the invasion of the mammalian host by viruses, bacteria, and parasites. TNF-alpha is an acute phase protein which initiates a cascade of cytokines and increases vascular permeability, thereby recruiting macrophage and neutrophils to a site of infection. TNF-alpha secreted by the macrophage causes blood clotting which serves to contain the infection. TNF-alpha has been detected in synovial fluid of patients with rheumatoid arthritis. Clone TN3-19.12 antibody can neutralize the bioactivity of natural or recombinant TNF-alpha
Recombinant mouse TNFalpha
0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.
>95% by SDS-PAGE and HPLC
>98% by SDS-PAGE and HPLC
≤ 1.0 EU/mg as determined by the LAL method
≤ 0.75 EU/mg as determined by the LAL method
Aggregation level ≤ 5%
Aggregation level ≤ 1%
We use the IMPACT test generated by IDEXX Laboratories to guarantee our Ultra Low Endotoxin antibodies are pathogen free. Our hamster antibodies are tested for:
Pneumonia virus of mice
Kilham’s rat virus
Toolan’s H1 virus
Minute virus of mice
Theiler’s murine encephalomyelitis virus
anti-TNF alpha In Vivo Antibody – Low Endotoxin (TN3-19.12) is stable for at least four (4) weeks when stored sterile at 2-8°C. For long-term storage aseptically aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Immunoprecipitation, Western Blot, Functional Assays, Flow Cytometry, Blocking
How much TN3-19.12 to use in vivo:
We recommend using 250 µg per mouse when performing in vivo; research using ichorbio’s low endotoxin TNF alpha antibody clone TN3-19.12. This range is based off the most recent publication data using the TN3-19.12 clone in vivo. Each investigator should determine their own optimal working dilution for specific applications.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
Immunofluorescence analysis of paraffin-embedded mouse liver tissue section labeling TNF-alpha (1:100 dilution) overnight at 4˚, followed by goat anti-hamster IgG H&L (Alexa Fluor ® 647-red) secondary antibody (1:500 dilution). The nuclear counter stain is DAPI (blue). Image was acquired on a Nikon A1R microscope system at 4x magnification (first image) or 60x magnification (second image).