Anti-Mouse PD-L1 In Vivo Antibody – Low Endotoxin (10F.9G2) [ICH1086]

p16-dependent upregulation of PD-L1 impairs immunosurveillance of senescent cells

ichorbio’s anti-PD-L1 In Vivo Antibody – Low Endotoxin (10F.9G2) is manufactured in a cGMP compliant facility. ichorbio’s low endotoxin antibodies have half the endotoxin of comparable antibodies from our competitors at less than 1.0 EU/mg. If ichorbio’s low endotoxin antibodies are not low enough we also offer ultra low endotoxin antibodies which have even less endotoxin (<0.75EU/mg) at an even higher purity (98% versus 95%). ichorbio: the best antibodies for in vivo research.

PD-L1

10F.9G2

ichorbio’s 10F.9G2 in vivo antibody is available in the following bulk sizes:
1mg, 5mg, 25mg, 50mg and 100mg
ichorbio regularly manufactures multi-gram amounts of our anti-PD-L1 10F.9G2 clone – please contact us for pricing.

Rat IgG2b

Programmed cell death 1 ligand 1, Cd274, PDCD1 ligand 1, Programmed death ligand 1, B7 homolog 1, B7-H1, B7h1, Pdcd1l1, Pdcd1lg1, Pdl1

Q9EP73

Rat

Mouse, Rat

Anti-PD-L1 In Vivo Antibody – Low Endotoxin (10F.9G2) recognizes an epitope on Mouse CD274

This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

The CD274 antigen is present on T cells, B cells, NK cells, dendritic cells, IFN-γ activated endothelial cells, and monocytes

CD274, also known as B7-H1 and PD-L1, is a 40 kD type I transmembrane protein and a member of the B7 family within the immunoglobulin receptor superfamily. It is expressed on T cells, B cells, NK cells, dendritic cells, IFN-γ activated endothelial cells, and monocytes.

Unknown

1.0 – 5.0 mg/ml

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.

>95% by SDS-PAGE and HPLC

>98% by SDS-PAGE and HPLC

<1.0 EU/mg as determined by the LAL method

<0.5 EU/mg as determined by the LAL method

Aggregation level ≤ 5%

Aggregation level ≤ 1%

This antibody is stable for at least 4 weeks when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 20°C or -80°C. Avoid repeated freeze thaw cycles.

Flow Cytometry, Blocking, IHC (Frozen), Immunofluorescence, Functional Assays

Each investigator should determine their own optimal working dilution for specific applications.

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

Rat IgG2b In Vivo Isotype Control – Low Endotoxin [1-2] (ICH2243)

Mouse anti-Mouse PD-L1 Antibody (10F.9G2) [ICH1183]

Sample: Frozen sections of tumor tissues from tumor bearing C57BL / 6 mice (inoculated with LLC cells)
Protocol:
1. Tumors were dissected, fixed in 4% paraformaldehyde, and dehydrated in 30% sucrose;
2. Frozen tumor sections were prepared at 25 ℃ and rinsed in PBS;
3. Blocking buffer: PBS containing 0.3% Triton + 5% goat serum; Sections were blocked for 1h;
4. Primary antibodies: Diluted in blocking buffer; incubated overnight at 4℃. Final concentration of ichorbio PD-L1 antibody clone 10F.9G2 (low) 1μg/ml, Final concentration (high) 5μg/ml. Positive signals were detected at both high and low concentrations
5. Washed by PBST; Secondary antibodies: incubated at 4℃ for 6h; DAPI: 2h
Details of secondary antibody:
Alexa Fluor 647-AffiniPure Goat Anti-Rat IgG (H+L) (min X Hu,Bov,Hrs Sr Prot) antibody – Jackson Immunoresearch Labs Cat# 112-605-062 – Conc. 7.5μg/ml.
6. Washed by PBST at least 6 times；
7. Add fluorescence decay resistant medium, seal slice;
8. Detected by the laser scanning confocal microscope.
Scale bar in the IF figure is 50 μM.
Images produced by Dr. Qin from State Key Laboratory of Genetic Engineering, School of Life Sciences of Fudan University