Anti-Mouse PD-1 In Vivo Antibody – Low Endotoxin (29F.1A12) [ICH1091]

ichorbio’s anti-PD-1 In Vivo Antibody – Low Endotoxin (29F.1A12) is manufactured in a cGMP compliant facility. Click here to view ichorbio’s complete list of anti-PD-1 antibodies and biosimilars. ichorbio: the best antibodies for in vivo research.

PD-1

29F.1A12

ichorbio’s 29F.1A12 in vivo antibody is available in the following bulk sizes:
1mg, 5mg, 25mg, 50mg and 100mg
ichorbio regularly manufactures multi-gram amounts of our anti-PD-1 29F.1A12 clone – please contact us for pricing.

Rat IgG2a

Programmed cell death protein 1, Pdcd1, CD279

Q02242

Rat

Mouse, Rat

Anti-PD-1 In Vivo Antibody – Low Endotoxin (29F.1A12) recognizes an epitope on Mouse PD-1. Despite its predicted molecular weight, PD-1 often migrates at higher molecular weight in SDS-PAGE.

This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Induced on splenic T and B lymphocytes, thymocytes, and myeloid cells after stimulation.Subset of double negative thymocytes, activated T and B cells

Programmed death-1 (PD-1), also know as CD279 is a 50-55 kD glycoprotein belonging to the CD28 family of the Ig superfamily. PD-1 is transiently expressed on CD4 and CD8 thymocytes as well as activated T and B lymphocytes and myeloid cells. Like the clones RMP1-14 and J43 antibodies, the 29F.1A12 antibody has been shown to block the binding of PD-1 to its ligands in vivo.

PD-1 cDNA followed by PD-1-Ig fusion protein

1.0 – 5.0 mg/ml

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.

>95% by SDS-PAGE and HPLC

>98% by SDS-PAGE and HPLC

≤ 1.0 EU/mg as determined by the LAL method

≤ 0.75 EU/mg as determined by the LAL method

Aggregation level ≤ 5%

Aggregation level ≤ 1%

This antibody is stable for at least 4 weeks when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 20°C or -80°C. Avoid repeated freeze thaw cycles.

Flow Cytometry, Western Blot, Blocking, IHC (Frozen)

Western Blotting: To detect Mouse PD-1 this antibody can be used at a concentration of 30 µg/ml.
Blocking: The 29F.1A12 antibody has been shown to block the binding of PD-1 to its ligands in vivo. For immune checkpoint antibodies 200 ug per mouse per injection is the standard dosage in the literature. NOTE: The response is model dependent. We have seen some tumor lines in mice that grow progressively. If we treat the animal with anti-PD-1 (days 3,6,9 @ 200 ug per animal per injection) then the tumor will regress, and disappear.
Each investigator should determine their own optimal working dilution for specific applications.

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

Rat IgG2a In Vivo Isotype Control – Low Endotoxin [1-1] (ICH2244)

Anti-PD-1 In Vivo Antibody – Low Endotoxin [RMP1-14] (ICH1132), Anti-PD-1 In Vivo Antibody – Ultra Low Endotoxin [RMP1-14] (ICH1132UL)