Bulk anti-Human MHC Class I antibody (W6/32) [ICH1023]

ICH1023 is up to 30% cheaper for academia & non-profits and up to 51% cheaper for industry than the equivalent product BE0079 from Bio X Cell. Product Benefits:

ichorbio's bulk anti-MHC Class I Antibody (W6/32) is manufactured in a cGMP compliant, ISO Quality Standard 9001:2015 facility. ichorbio's low endotoxin antibodies have half the endotoxin of comparable antibodies from our competitors at less than 1.0 EU/mg. If ichorbio's low endotoxin antibodies are not low enough we also offer ultra low endotoxin antibodies which have even less endotoxin (<0.75EU/mg) at an even higher purity (98% versus 95%). ichorbio: the best antibodies for in vivo research.

Target:

MHC Class I

Clone:

W6/32

Size:

ichorbio's W6/32 in vivo antibody is available in the following bulk sizes: 1mg, 5mg, 25mg, 50mg and 100mg ichorbio regularly manufactures multi-gram amounts of our anti-MHC Class I W6/32 clone - please contact us for pricing.

Isotype:

Mouse IgG2a

Other Names:

Major Histocompatibility Class I, MHC class I

Host:

Mouse

Species Reactivity:

Human

Specificity:

Bulk anti-human MHC Class I antibody (W6/32) recognizes/reacts with major histocompatibility complex (MHC) class I, HLA-A, B, and C

Purification Method:

This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Antigen Distribution:

This HLA-A, B, C antigen is present on most nucleated cells of humans and old world monkeys.

Background:

MHC class I antigens associated with β2-microglobulin are expressed by all human nucleated cells. MHC class I molecules are involved in the presentation of antigens to CD8+ T cells and plays a central role in cell-mediated immune responses and tumor surveillance.

Immunogen:

Human tonsil cell membrane

Concentration:

1.0 mg/ml

Formulation:

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.

Purity:

>95% by SDS-PAGE and HPLC

>98% by SDS-PAGE and HPLC

Endotoxin:

≤ 1.0 EU/mg as determined by the LAL method

≤ 0.75 EU/mg as determined by the LAL method

Aggregation:

Aggregation level ≤ 5%

Aggregation level ≤ 1%

Storage:

This antibody is stable for at least 4 weeks when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 20°C or -80°C. Avoid repeated freeze thaw cycles.

Applications:

Western Blot, Flow Cytometry, Immunoprecipitation, Functional Assays, IHC (Frozen)

Application Notes:

Each investigator should determine their own optimal working dilution for specific applications.

Use:

Products are for research use only.

Isotype Control:

Mouse IgG2a In Vivo Isotype Control - Low Endotoxin [C1.18.4] (ICH2245)

Antibodies against the same target:

Anti-MHC Class I In Vivo Antibody - Low Endotoxin [A4C8.1-Do9] (ICH1097)