Bulk anti-GM-CSF In Vivo Antibody – Low Endotoxin (MP1-22E9)
ichorbio’s anti-GM-CSF In Vivo Antibody – Low Endotoxin (MP1-22E9) is manufactured in a cGMP compliant facility. ichorbio’s low endotoxin antibodies have half the endotoxin of comparable antibodies from our competitors at less than 1.0 EU/mg. If ichorbio’s low endotoxin antibodies are not low enough we also offer ultra low endotoxin antibodies which have even less endotoxin (<0.75EU/mg) at an even higher purity (98% versus 95%). ichorbio: the best antibodies for in vivo research.
ichorbio’s MP1-22E9 in vivo antibody is available in the following bulk sizes:
1mg, 5mg, 25mg, 50mg and 100mg
ichorbio regularly manufactures multi-gram amounts of our anti-GM-CSF MP1-22E9 clone – please contact us for pricing.
Csf2, Granulocyte-macrophage colony-stimulating factor, Colony-stimulating factor, CSF, Csfgm
Anti-GM-CSF In Vivo Antibody – Low Endotoxin (MP1-22E9) recognizes an epitope on Mouse GM-CSF
This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype
Granulocyte-macrophage colony-stimulating factor, often abbreviated to GM-CSF is a pleiotropic cytokine that controls the production and function of blood cells. GM-CSF stimulates stem cells to produce granulocytes (neutrophils, eosinophils, and basophils) and monocytes.GM-CSF has a critical role in regulation of surfactant homeostasis and alveolar macrophage innate immune functions in the lung.
Yeast Derived Recombinant Mouse GM-CSF
1.0 – 5.0 mg/ml
0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. BSA and Azide free.
>95% by SDS-PAGE and HPLC
>98% by SDS-PAGE and HPLC
≤ 1.0 EU/mg as determined by the LAL method
≤ 0.75 EU/mg as determined by the LAL method
Aggregation level ≤ 5%
Aggregation level ≤ 1%
We use the IMPACT test generated by IDEXX Laboratories to guarantee our Ultra Low Endotoxin antibodies are pathogen free. Our rat antibodies are tested for:
Pneumonia virus of mice
Kilham’s rat virus
Toolan’s H1 virus
Lymphocytic choriomeningitis virus
Rat minute virus
anti-GM-CSF In Vivo Antibody – Low Endotoxin (MP1-22E9) is lyophilized and can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Western Blot, ELISA, ELISPOT, Blocking, Immunocytochemistry, IHC (Frozen)
Western Blotting: To detect Mouse GM-CSF this monoclonal antibody can be used at a concentration of 1-2 µg/ml.Each investigator should determine their own optimal working dilution for specific applications.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
Sample: Frozen sections of tumor tissues from tumor bearing C57BL / 6 mice (inoculated with LLC cells)
1. Tumors were dissected, fixed in 4% paraformaldehyde, and dehydrated in 30% sucrose;
2. Frozen tumor sections were prepared at 25 ℃ and rinsed in PBS;
3. Blocking buffer: PBS containing 0.3% Triton + 5% goat serum; Sections were blocked for 1h;
4. Primary antibodies: Diluted in blocking buffer; incubated overnight at 4℃. Final concentration of ichorbio GM-CSF antibody clone MP1-22E9 (low) 2.7μg/ml, Final concentration (high) 13.5μg/ml. Positive signals were detected at both high and low concentrations
5. Washed by PBST; Secondary antibodies: incubated at 4℃ for 6h; DAPI: 2h
Details of secondary antibody:
Alexa Fluor 647-AffiniPure Goat Anti-Rat IgG (H+L) (min X Hu,Bov,Hrs Sr Prot) antibody – Jackson Immunoresearch Labs Cat# 112-605-062 – Conc. 7.5μg/ml.
6. Washed by PBST at least 6 times；
7. Add fluorescence decay resistant medium, seal slice；
8. Detected by the laser scanning confocal microscope.
Scale bar in the IF figure is 50 μM.
Images produced by Dr. Qin from State Key Laboratory of Genetic Engineering, School of Life Sciences of Fudan University