Bulk anti-CTLA-4 In Vivo Antibody – Low Endotoxin (9D9)
ichorbio’s anti-CTLA-4 In Vivo Antibody – Low Endotoxin (9D9) is manufactured in a cGMP compliant, ISO Quality Standard 9001:2015 facility. ichorbio’s low endotoxin antibodies have half the endotoxin of comparable antibodies from our competitors at less than 1.0 EU/mg. If ichorbio’s low endotoxin antibodies are not low enough we also offer ultra low endotoxin antibodies which have even less endotoxin (<0.75EU/mg) at an even higher purity (98% versus 95%). ichorbio: the best antibodies for in vivo research.
ichorbio’s 9D9 in vivo antibody is available in the following bulk sizes:
1mg, 5mg, 25mg, 50mg and 100mg
ichorbio regularly manufactures multi-gram amounts of our anti-CTLA-4 9D9 clone – please contact us for pricing.
Ctla4, Cytotoxic T-lymphocyte protein 4, CD152
Anti-CTLA-4 In Vivo Antibody – Low Endotoxin (9D9) recognizes an epitope on Mouse CTLA-4
This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Activated T cells
Negative regulator of T cell activation
1.0 – 5.0 mg/ml
0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.
>95% by SDS-PAGE and HPLC
>98% by SDS-PAGE and HPLC
≤ 1.0 EU/mg as determined by the LAL method
≤ 0.75 EU/mg as determined by the LAL method
Aggregation level ≤ 5%
Aggregation level ≤ 1%
We use the IMPACT test generated by IDEXX Laboratories to guarantee our Ultra Low Endotoxin antibodies are pathogen free. Our mouse antibodies are tested for:
Mouse hepatitis virus
Pneumonia virus of mice
Minute virus of mice
Mouse parvovirus (MPV1-5)
Theiler’s murine encephalomyelitis virus
Lymphocytic choriomeningitis virus
Lactate dehydrogenase-elevating virus
Mouse adenovirus (MAD1, MAD2)
Mouse thymic virus
Corynebacterium spp. (HAC2)
anti-CTLA-4 In Vivo Antibody – Low Endotoxin (9D9) is stable when stored at 2-8°C for at least four (4) weeks. For long term storage aseptically aliquot into working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
Western Blot, Functional Assays
We recommend using between 50-250µg per mouse when performing in vivo research using ichorbio’s CTLA-4 low endotoxin antibody clone 9D9. This range is based off the most recent publication data using the 9D9 clone in vivo.
Each investigator should determine their own optimal working dilution for specific applications.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.