Anti-CD80 In Vivo Antibody – Low Endotoxin (16-10A1) [ICH1037]

ichorbio’s anti-CD80 In Vivo Antibody – Low Endotoxin (16-10A1) is manufactured in a cGMP compliant facility. ichorbio’s low endotoxin antibodies have half the endotoxin of comparable antibodies from our competitors at less than 1.0 EU/mg. If ichorbio’s low endotoxin antibodies are not low enough we also offer ultra low endotoxin antibodies which have even less endotoxin (<0.75EU/mg) at an even higher purity (98% versus 95%). ichorbio: the best antibodies for in vivo research.

CD80

16-10A1

ichorbio’s 16-10A1 in vivo antibody is available in the following bulk sizes:
1mg, 5mg, 25mg, 50mg and 100mg
ichorbio regularly manufactures multi-gram amounts of our anti-CD80 16-10A1 clone – please contact us for pricing.

Armenian Hamster IgG

Activation B7-1 antigen, B7

Q00609

Armenian Hamster

Mouse, Rat

Anti-CD80 In Vivo Antibody – Low Endotoxin (16-10A1) recognizes an epitope on Mouse CD80

This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Mouse CD80 is expressed on Activated B-Cells, Activated T-Cells, macrophages, and dendritic cells.

CD80 (B7-1) is a type I integral membrane protein related very closely to CD86 (B7-2). A main function of CD80 is the costimulation of T-cells through ligation of CD28 and CD152 (CTLA-4). T-cells activate with binding of CD28 to CD80 and binding of CD80 to CTLA-4 results in inhibition of T-cells. The clone 16-10A1 has the ability to block the costimulatory activity of CD80 while exhibiting no effect on the activity of CD86.

Purified Recombinant Mouse CD80 (>98%)

1.0 mg/ml

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.

>95% by SDS-PAGE and HPLC

>98% by SDS-PAGE and HPLC

≤ 1.0 EU/mg as determined by the LAL method

≤ 0.75 EU/mg as determined by the LAL method

Aggregation level ≤ 5%

Aggregation level ≤ 1%

This antibody is stable for at least 4 weeks when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 20°C or -80°C. Avoid repeated freeze thaw cycles.

Immunoprecipitation, Western Blot, Blocking, Flow Cytometry, IHC (Frozen)

Each investigator should determine their own optimal working dilution for specific applications.

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

Armenian Hamster IgG Isotype Control for In Vivo – Low Endotoxin [PIP] (ICH2251)