Anti-Mouse CD172a In Vivo Antibody – Low Endotoxin (P84) [ICH1076]

ichorbio’s anti-CD172a In Vivo Antibody – Low Endotoxin (P84) is manufactured in a cGMP compliant, ISO Quality Standard 9001:2015 facility. ichorbio’s low endotoxin antibodies have half the endotoxin of comparable antibodies from our competitors at less than 1.0 EU/mg. If ichorbio’s low endotoxin antibodies are not low enough we also offer ultra low endotoxin antibodies which have even less endotoxin (<0.75EU/mg) at an even higher purity (98% versus 95%). ichorbio: the best antibodies for in vivo research.

CD172a

P84

Rat IgG1

Sirpa, Sirp-alpha-1, SHP substrate 1, SHPS-1, Shps1, Bit, p84, MyD-1 antigen, Myd1, Ptpns1

P97797

Rat

Mouse, Rat

Anti-CD172a In Vivo Antibody – Low Endotoxin (P84) recognizes an epitope on Mouse CD172a

This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Monocytes, macrophages, myeloid cells, neuronal tissue

Signal regulatory protein alpha (SIRPalpha), also known as CD172a, is a regulatory membrane type I glycoprotein from the SIRP family expressed mainly by myeloid cells and also by stem cells or neurons.

Mouse brain membrane protein

1.0 – 5.0 mg/ml

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.

>95% by SDS-PAGE and HPLC

>98% by SDS-PAGE and HPLC

≤ 1.0 EU/mg as determined by the LAL method

≤ 0.75 EU/mg as determined by the LAL method

Aggregation level ≤ 5%

Aggregation level ≤ 1%

This antibody is stable for at least 4 weeks when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 20°C or -80°C. Avoid repeated freeze thaw cycles.

Western Blot, Blocking, Flow Cytometry, IHC (Frozen), Immunoprecipitation

Blocking: Reported applications in literature include: blocking SIRPalpha interaction with CD47 & in vivo blocking of dendritic cell migration.
Each investigator should determine their own optimal working dilution for specific applications.

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

Rat IgG1 Isotype Control for In Vivo – Low Endotoxin [GL113] (ICH2246)