9H10 and Isotype Control
This product is only available in the USA.
This product includes two antibodies – primary antibody 9H10 (ICH1084) plus its matching isotype control (ICH2253)
ichorbio’s 9H10 antibody is available in the following sizes: 5mg, 25mg, 50mg and 100mg.
This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
1.0 – 5.0 mg/ml
0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.
>95% by SDS-PAGE and HPLC
>98% by SDS-PAGE and HPLC
≤ 1.0 EU/mg as determined by the LAL method
≤ 0.75 EU/mg as determined by the LAL method
Aggregation level ≤ 5%
Aggregation level ≤ 1%
IMPACT Pathogen Test:
We use the IMPACT test generated by IDEXX Laboratories to guarantee our Ultra Low Endotoxin antibodies are pathogen free. Our rat antibodies are tested for: Mycoplasma spp., Mycoplasma pulmonis, Pneumonia virus of mice, Kilham’s rat virus, Toolan’s H1 virus, Rat parvovirus, Lymphocytic choriomeningitis virus, Rat cytomegalovirus, Sendai virus, Rat coronavirus, Sialodacryoadenitis virus, Rat minute virus, Seoul virus, Mouse adenovirus, Reovirus 3, Rat theilovirus
Anti-PD-L1 In Vivo Antibody – Low Endotoxin (10F.9G2) is stable when stored at 2-8°C. For longer term storage (> 3 months) it is recommended to store this antibody at -70oC.
Western Blot, Blocking, Flow Cytometry, Functional Assays
Blocking: We recommend using between 50-250µg when performing in vivo research using ichorbio’s CTLA-4 low endotoxin antibody clone 9H10. This range is based off the most recent publication data using the 9H10 clone in vivo. Each investigator should determine their own optimal working dilution for specific applications.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.