With the help of our partner, Binaree – ichorbio has been creating new immunofluorescence validation data for a number of our antibodies. One of these antibodies is anti-Ly-6G antibody, clone 1A8
Immunofluorescence Staining Protocol:
Immunofluorescence analysis of paraffin-embedded mouse spleen tissue section labeling Ly-6g (1:100 dilution) overnight at 4˚, followed by goat anti-mouse IgG H&L (Alexa Fluor ® 647-red) secondary antibody (1:500 dilution). The nuclear counter stain is DAPI (blue).
Image was acquired on a Nikon A1R microscope system at 4x magnification (100um panel) or 60x magnification (20um panel).
Additional immunoflourescence validation images for clone TN3-19.12 are below: