Bulk anti-HLA antibodies

ichorbio supplies a range of HLA antibodies including antibodies targeting DR, DQ and DP isotypes. We have low endotoxin antibodies available for each which are suitable for in vivo use.

Anti-HLA-DR Antibodies

ichorbio supplies clone L243 as our anti-HLA-DR antibody. Clone L243 is available in bulk quantities as standard – 5mg-100mg. We also have gram quantities readily available so please send your enquiry to [email protected].

 

HLA-DR

HLA-DR is an isotype of MHC class II together with HLA-DP, HLA-DM, HLA-DO and HLA-DQ

HLA-DR is encoded on chromosome 6 in the region 6p21; the DR isotype is located specifically at 6p21.31. HLA-DR is made up of two subunits: alpha (α) and beta (β).

MHC Class II

MHC Class II proteins are up-regulated in response to signalling as part of the immune response. They form a complex with foreign peptides generally 9 to 30 amino acids in length on the outside of cells which together act as a ligand for the T-cell receptor (TCR) which causes the multiplication of T-helper cells (also called CD4 positive T cells).  These cells in turn stimulate antibody-producing B-cells to produce antibodies to the specific foreign peptide that was presented.

Please see image 1 below for a schematic:

HLA-DR in Disease

Antigens most responsible for graft loss are HLA-DR (first six months), HLA-B (first two years), and HLA-A (long-term survival). Good matching of these antigens between host and donor is most critical for achieving graft survival.

HLA-DR is also involved in several autoimmune conditions, disease susceptibility and disease resistance. It is also closely linked to HLA-DQ and this linkage often makes it difficult to resolve the more causative factor in disease.

HLA DR alleles that are associated with autoimmune diseases:

Anti-HLA-DQ antibodies

ichorbio supplies clone 1a3 as our anti-HLA-DQ antibody. Clone 1a3 is available in bulk quantities with standard sizes 5mg-100mg. We also frequently manufacture gram quantities of clone 1a3.

 

HLA-DQ antibodies: Low Endotoxin

HLA-DQ

HLA-DQ is an isotype of MHC class II together with HLA-DP, HLA-DM, HLA-DO and HLA-DR

HLA-DQ is encoded on chromosome 6 in the region 6p21; the DQ isotype is located specifically at 6p21.31. HLA-DQ is made up of two subunits: alpha (α) and beta (β).

The currently used serotypes are HLA-DQ2, -DQ3, -DQ4, -DQ5, -DQ6, -DQ7, -DQ8, -DQ9.

MHC Class II

MHC Class II proteins are up-regulated in response to signalling as part of the immune response. They form a complex with foreign peptides generally 9 to 30 amino acids in length on the outside of cells which together act as a ligand for the T-cell receptor (TCR) which causes the multiplication of T-helper cells (also called CD4 positive T cells).  These cells in turn stimulate antibody-producing B-cells to produce antibodies to the specific foreign peptide that was presented.

Please see image 1 below for a schematic:

DQ is also involved in recognizing common self-antigens and presenting those antigens to the immune system in order to develop tolerance from a very young age. When tolerance to self proteins is lost, DQ may become involved in autoimmune disease.

HLA-DQ in Disease

Two autoimmune diseases in which HLA-DQ is involved are coeliac disease and diabetes mellitus type 1.

DQ is one of several antigens involved in rejection of organ transplants. As a variable cell surface receptor on immune cells, these D antigens, originally HL-A4 antigens, are involved in graft-versus-host disease when lymphoid tissues are transplanted between people.

Anti-HLA-DP antibodies

ichorbio supplies clone B7/21 as our anti-HLA-DP antibody. Clone B7/21 is available in bulk quantities with standard sizes 5mg-100mg. We also frequently manufacture gram quantities of clone B7/21.

 

HLA-DP antibody clone B7/21: Low Endotoxin