What is 10F.9G2?

RMP1-14 is specific clone name for a rat anti-mouse PD-1 antibody. The original hybridoma was generated by immunizing Sprague Dawley rats with mouse PD-1-transfected BHK cells. A P3U1 myeloma was used as the fusion partner.

Who was it created by?

It was developed by Michael Eppihimer at the Wyeth/Genetics Institute

Where was it first published?

Authors: Eppihimer et al.

Journal Title: Expression and Regulation of the PD-L1 Immunoinhibitory Molecule on Microvascular Endothelial Cells.

Journal: Microcirculation

Additional Info: 2002 Apr;9(2):133-45. PMID:11932780. Describes the original generation of this antibody, and its use in ELISA, flow cytometry and immunohistochemistry.

Blocking Functionality

The 10F.9G2 antibody has been shown to block the interaction between PD-L1 and PD-1 and between PD-L1 and B7-1 (CD80).

Citations

A total of 331 articles have been published over the last three years citing 10F.9G2, with scientists from 210 organisations in 26 countries contributing to the research. The top authors publishing articles using 10F.9G2 in the last three years are show in Table 1 below.

Table 1: The top 10 most prolific authors citing 10F.9G2 in the last 3 years

NameUniversityCountry
Wen-Fang ChengNational Taiwan UniversityTaiwan
Chien-An ChenNational Taiwan UniversityTaiwan
Kabir KhanUniversity of TorontoCanada
Dirk SchadendorfUniversity of Duisburg-EssenGermany
Payal DharNorthwestern UniversityUnited States
Claudia de LorenzoUniversity of Naples Federico IIItaly
Jun TangSun Yat-sen UniversityChina
Xiao-Jing WangUniversity of California DavisUnited States
Jing WangUniversity of PittsburghUnited States
Yang-Xin FuTsinghua UniversityChina

Data from Pubmed: the National Library for Medicine

A breakdown of the main locations of the authors are shown in Image 1 and 2 below. In Image 3 we show the main journals that cite work using RMP1-14.

Image 1: Citations by Country

Countries citing 10F.9G2 the most

Image 2: Citations by State (USA)

States citing 10F.9G2 the most

Image 3: Citations by Journal

Journals citing 10F.9G2 the most

Images kindly provided by SciLeads

Latest Research Articles Using 10F.9G2

  1. Jae Kim, Korea University of Science and Technology. Improving anticancer effect of aPD-L1 through lowering neutrophil infiltration by PLAG in tumor implanted with MB49 mouse urothelial carcinoma.
  2. Atsushi Enomoto, Nagoya University. Meflin-positive cancer-associated fibroblasts enhance tumor response to immune checkpoint blockade.
  3. Yun Liang, Zhejiang University. Inhibition of myeloid-derived suppressive cell function with all-trans retinoic acid enhanced anti-PD-L1 efficacy in cervical cancer.

ichorbio has created a number of antibodies using the 10F.9G2 clone:

Matching isotype controls: